鲍曼不动杆菌中因ISAba1插入导致头孢菌素酶过表达。
Cephalosporinase over-expression resulting from insertion of ISAba1 in Acinetobacter baumannii.
作者信息
Héritier C, Poirel L, Nordmann P
机构信息
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, Le Kremlin-Bicêtre, France.
出版信息
Clin Microbiol Infect. 2006 Feb;12(2):123-30. doi: 10.1111/j.1469-0691.2005.01320.x.
ISAba1-like sequences were identified immediately upstream of the bla(ampC) gene in ceftazidime-resistant Acinetobacter baumannii isolates, but were absent in ceftazidime-susceptible A. baumannii isolates. AmpC over-expression resulted from insertion of ISAba1-like sequences upstream of bla(ampC). ISAba1 provided strong promoter sequences, and it was demonstrated that the change in the ribosome binding site sequence resulting from insertion of ISAba1 did not influence expression of the bla(ampC) gene. Sequence analysis revealed that AmpC sequences of A. baumannii isolates were almost identical and that ISAba1 elements had a high percentage of identity.
在对头孢他啶耐药的鲍曼不动杆菌分离株中，在bla(ampC)基因上游紧邻处鉴定出ISAba1样序列，但在对头孢他啶敏感的鲍曼不动杆菌分离株中未发现该序列。AmpC的过表达是由于ISAba1样序列插入到bla(ampC)基因上游所致。ISAba1提供了强启动子序列，并且已证明因ISAba1插入导致的核糖体结合位点序列变化不影响bla(ampC)基因的表达。序列分析显示，鲍曼不动杆菌分离株的AmpC序列几乎相同，且ISAba1元件具有很高的同一性百分比。
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