• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

益生菌KUB-AC5的整合生长生理学与转录组分析

Integrative growth physiology and transcriptome profiling of probiotic KUB-AC5.

作者信息

Jatuponwiphat Theeraphol, Namrak Thanawat, Nitisinprasert Sunee, Nakphaichit Massalin, Vongsangnak Wanwipa

机构信息

Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok, Thailand.

Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand.

出版信息

PeerJ. 2021 Oct 5;9:e12226. doi: 10.7717/peerj.12226. eCollection 2021.

DOI:10.7717/peerj.12226
PMID:34707932
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8500091/
Abstract

KUB-AC5 has been widely used as probiotic in chicken for reduction. However, a preferable carbon source and growth phase is poorly characterized underlying metabolic responses on growth and inhibition effects of KUB-AC5. This study therefore aimed to investigate transcriptome profiling of KUB-AC5 revealing global metabolic responses when alteration of carbon sources and growth phases. Interestingly, KUB-AC5 grown under sucrose culture showed to be the best for fast growth and inhibition effects against Enteritidis S003 growth. Towards the transcriptome profiling and reporter proteins/metabolites analysis, the results showed that amino acid transport ABC systems as well as sucrose metabolism and transport are key metabolic responses at Logarithmic (L)-phase of KUB-AC5 growth. Considering the Stationary (S)-phase, we found the potential reporter proteins/metabolites involved in carbohydrate metabolism ., levansucrase and levan. Promisingly, levansucrase and levan were revealed to be candidates in relation to inhibition effects of KUB-AC5. Throughout this study, KUB-AC5 had a metabolic control in acclimatization to sucrose and energy pools through transcriptional co-regulation, which supported the cell growth and inhibition potentials. This study offers a perspective in optimizing fermentation condition through either genetic or physiological approaches for enhancing probiotic KUB-AC5 properties.

摘要

KUB - AC5已被广泛用作鸡的益生菌以实现减少（某种情况，原文未明确）。然而，关于KUB - AC5在生长和抑制作用方面的潜在代谢反应，其优选的碳源和生长阶段尚未得到充分表征。因此，本研究旨在调查KUB - AC5的转录组图谱，以揭示碳源和生长阶段改变时的全局代谢反应。有趣的是，在蔗糖培养条件下生长的KUB - AC5在快速生长和对肠炎沙门氏菌S003生长的抑制作用方面表现最佳。对于转录组图谱分析以及报告蛋白/代谢物分析，结果表明氨基酸转运ABC系统以及蔗糖代谢和转运是KUB - AC5生长对数期（L期）的关键代谢反应。考虑到稳定期（S期），我们发现了参与碳水化合物代谢的潜在报告蛋白/代谢物，即果聚糖蔗糖酶和果聚糖。有希望的是，果聚糖蔗糖酶和果聚糖被揭示为与KUB - AC5抑制作用相关的候选物。在整个研究过程中，KUB - AC5通过转录共调节在适应蔗糖和能量库方面具有代谢控制，这支持了细胞生长和抑制潜力。本研究为通过遗传或生理方法优化发酵条件以增强益生菌KUB - AC5特性提供了一个视角。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b6/8500091/007f225e5299/peerj-09-12226-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b6/8500091/3d68006a5e78/peerj-09-12226-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b6/8500091/c281d33aa2ea/peerj-09-12226-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b6/8500091/18e8c00bb6f3/peerj-09-12226-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b6/8500091/ef3a0fa4164f/peerj-09-12226-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b6/8500091/f2db59825964/peerj-09-12226-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b6/8500091/007f225e5299/peerj-09-12226-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b6/8500091/3d68006a5e78/peerj-09-12226-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b6/8500091/c281d33aa2ea/peerj-09-12226-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b6/8500091/18e8c00bb6f3/peerj-09-12226-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b6/8500091/ef3a0fa4164f/peerj-09-12226-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b6/8500091/f2db59825964/peerj-09-12226-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b6/8500091/007f225e5299/peerj-09-12226-g006.jpg

相似文献

1
Integrative growth physiology and transcriptome profiling of probiotic KUB-AC5.益生菌KUB-AC5的整合生长生理学与转录组分析
PeerJ. 2021 Oct 5;9:e12226. doi: 10.7717/peerj.12226. eCollection 2021.
2
Probing Genome-Scale Model Reveals Metabolic Capability and Essential Nutrients for Growth of Probiotic KUB-AC5.探索基因组规模模型揭示了益生菌KUB-AC5生长的代谢能力和必需营养素。
Biology (Basel). 2022 Feb 11;11(2):294. doi: 10.3390/biology11020294.
3
Synergistic activity of Limosilactobacillus reuteri KUB-AC5 and water-based plants against Salmonella challenge in a human in vitro gut model.植物源水提物与雷特氏乳杆菌 KUB-AC5 的协同作用对体外模拟人肠道中沙门氏菌感染的影响。
Sci Rep. 2024 Feb 27;14(1):4730. doi: 10.1038/s41598-024-53912-5.
4
Protective effect of Lactobacillus reuteri KUB-AC5 against Salmonella Enteritidis challenge in chickens.罗特氏乳杆菌 KUB-AC5 对鸡感染肠炎沙门氏菌的保护作用。
Benef Microbes. 2019 Feb 8;10(1):43-54. doi: 10.3920/BM2018.0034. Epub 2018 Nov 8.
5
Development of high cell density Limosilactobacillus reuteri KUB-AC5 for cell factory using oxidative stress reduction approach.采用降低氧化应激策略开发高细胞密度罗伊氏乳杆菌 KUB-AC5 细胞工厂。
Microb Cell Fact. 2023 Apr 29;22(1):86. doi: 10.1186/s12934-023-02076-4.
6
Probiotic KUB-AC5 decreases urothelial cell invasion and enhances macrophage killing of uropathogenic study.益生菌 KUB-AC5 可降低尿路上皮细胞侵袭性并增强巨噬细胞对尿路致病性细菌的杀伤作用。
Front Cell Infect Microbiol. 2024 Jul 18;14:1401462. doi: 10.3389/fcimb.2024.1401462. eCollection 2024.
7
Anti-inflammatory Effect of Probiotic KUB-AC5 Against Infection in a Mouse Colitis Model.益生菌KUB - AC5对小鼠结肠炎模型感染的抗炎作用
Front Microbiol. 2021 Aug 23;12:716761. doi: 10.3389/fmicb.2021.716761. eCollection 2021.
8
Antimicrobial peptide presenting potential strain-specific real time polymerase chain reaction assay for detecting the probiotic Lactobacillus reuteri KUB-AC5 in chicken intestine.用于检测鸡肠道中益生菌罗伊氏乳杆菌 KUB-AC5 的潜在菌株特异性实时聚合酶链反应检测的抗菌肽。
Poult Sci. 2020 Jan;99(1):526-535. doi: 10.3382/ps/pez549. Epub 2019 Dec 30.
9
The effect of including Lactobacillus reuteri KUB-AC5 during post-hatch feeding on the growth and ileum microbiota of broiler chickens.在出壳后饲养期间添加罗伊氏乳杆菌 KUB-AC5 对肉鸡生长和回肠微生物群的影响。
Poult Sci. 2011 Dec;90(12):2753-65. doi: 10.3382/ps.2011-01637.
10
Comparative genomics-based probiotic relevance of Limosilactobacillus fermentum KUB-D18.基于比较基因组学的发酵乳杆菌KUB-D18的益生菌相关性
Gene. 2022 Oct 5;840:146747. doi: 10.1016/j.gene.2022.146747. Epub 2022 Jul 18.

引用本文的文献

1
Genome-wide transcriptomics revealed carbon source-mediated gamma-aminobutyric acid (GABA) production in a probiotic, 9D3.全基因组转录组学揭示了益生菌9D3中碳源介导的γ-氨基丁酸（GABA）产生。
Heliyon. 2025 Jan 10;11(2):e41879. doi: 10.1016/j.heliyon.2025.e41879. eCollection 2025 Jan 30.
2
Growth promotion effect of red ginseng dietary fiber to probiotics and transcriptome analysis of .红参膳食纤维对益生菌的促生长作用及转录组分析
J Ginseng Res. 2023 Jan;47(1):159-165. doi: 10.1016/j.jgr.2022.09.003. Epub 2022 Oct 3.
3
Probing Genome-Scale Model Reveals Metabolic Capability and Essential Nutrients for Growth of Probiotic KUB-AC5.

本文引用的文献

1
Bacteriocins as a new generation of antimicrobials: toxicity aspects and regulations.细菌素作为新一代抗菌药物：毒性方面与法规。
FEMS Microbiol Rev. 2021 Jan 8;45(1). doi: 10.1093/femsre/fuaa039.
2
Antimicrobial peptide presenting potential strain-specific real time polymerase chain reaction assay for detecting the probiotic Lactobacillus reuteri KUB-AC5 in chicken intestine.用于检测鸡肠道中益生菌罗伊氏乳杆菌 KUB-AC5 的潜在菌株特异性实时聚合酶链反应检测的抗菌肽。
Poult Sci. 2020 Jan;99(1):526-535. doi: 10.3382/ps/pez549. Epub 2019 Dec 30.
3
Probiotic Properties of Lactobacilli and Their Ability to Inhibit the Adhesion of Enteropathogenic Bacteria to Caco-2 and HT-29 Cells.
探索基因组规模模型揭示了益生菌KUB-AC5生长的代谢能力和必需营养素。
Biology (Basel). 2022 Feb 11;11(2):294. doi: 10.3390/biology11020294.
乳酸菌的益生菌特性及其抑制肠致病性细菌对 Caco-2 和 HT-29 细胞黏附的能力。
Probiotics Antimicrob Proteins. 2021 Feb;13(1):102-112. doi: 10.1007/s12602-020-09659-2.
4
Protective effect of Lactobacillus reuteri KUB-AC5 against Salmonella Enteritidis challenge in chickens.罗特氏乳杆菌 KUB-AC5 对鸡感染肠炎沙门氏菌的保护作用。
Benef Microbes. 2019 Feb 8;10(1):43-54. doi: 10.3920/BM2018.0034. Epub 2018 Nov 8.
5
Biosynthesis of levan from sucrose using a thermostable levansucrase from Lactobacillus reuteri LTH5448.利用植物乳杆菌 LTH5448 的热稳定莱鲍迪苷蔗糖酶从蔗糖生物合成莱鲍迪苷。
Int J Biol Macromol. 2018 Jul 1;113:29-37. doi: 10.1016/j.ijbiomac.2018.01.187. Epub 2018 Feb 6.
6
Isolation and characterization of probiotics from dairies.乳制品中益生菌的分离与鉴定
Iran J Microbiol. 2017 Aug;9(4):234-243.
7
Transcriptomics technologies.转录组学技术
PLoS Comput Biol. 2017 May 18;13(5):e1005457. doi: 10.1371/journal.pcbi.1005457. eCollection 2017 May.
8
Current state of purification, isolation and analysis of bacteriocins produced by lactic acid bacteria.乳酸菌所产细菌素的纯化、分离和分析的现状。
Appl Microbiol Biotechnol. 2017 Feb;101(4):1323-1335. doi: 10.1007/s00253-017-8088-9. Epub 2017 Jan 9.
9
Beneficial Properties of Probiotics.益生菌的有益特性。
Trop Life Sci Res. 2016 Aug;27(2):73-90. doi: 10.21315/tlsr2016.27.2.6.
10
KEGG as a reference resource for gene and protein annotation.KEGG作为基因和蛋白质注释的参考资源。
Nucleic Acids Res. 2016 Jan 4;44(D1):D457-62. doi: 10.1093/nar/gkv1070. Epub 2015 Oct 17.