Burley Glenn A, Gierlich Johannes, Mofid Mohammad R, Nir Hadar, Tal Shay, Eichen Yoav, Carell Thomas
Department of Chemistry, Technion, Haifa, Israel.
J Am Chem Soc. 2006 Feb 8;128(5):1398-9. doi: 10.1021/ja055517v.
Genes of interest can be selectively metallized via the incorporation of modified triphosphates. These triphosphates bear functions that can be further derivatized with aldehyde groups via the use of click chemistry. Treatment of the aldehyde-labeled gene mixture with the Tollens reagent, followed by a development process, results in the selective metallization of the gene of interest in the presence of natural DNA strands.
通过掺入修饰的三磷酸可以对感兴趣的基因进行选择性金属化。这些三磷酸带有可通过点击化学用醛基进一步衍生化的功能。用托伦试剂处理醛标记的基因混合物，然后进行显影过程，可在天然DNA链存在的情况下实现对感兴趣基因的选择性金属化。
