Oliveira C A, Germano P M, Bird C, Pinto C A
Departamento de Produção Animal (VPA), Faculdade de Medicina Veterináría e Zootecnia, Universidade de São Paulo, Brazil.
Food Addit Contam. 1997 Jan;14(1):7-10. doi: 10.1080/02652039709374491.
Aflatoxin M1 was surveyed in 300 samples of whole milk powder consumed by infants at municipal schools and nurseries in São Paulo, Brazil. The analyses were performed by using commercially available test systems of a direct competitive enzyme-linked immunosorbent assay (ELISA). Samples were reconstituted in water (1:8), centrifuged at 1630 x gav for 15 min, and submitted directly to the assay without clean-up procedures. Results showed 33 (11%) positive samples for aflatoxin M1 at levels of 0.10-1.00 ng/ml (mean: 0.27 +/- 0.20 ng/ml). By using data on milk consumption patterns for 4-month-old children (highest intake), a mean daily intake of 3.7 ng/kg body weight/day was estimated. The implications of these data on human health are discussed.
对巴西圣保罗市立学校和托儿所中婴儿食用的300份全脂奶粉样本进行了黄曲霉毒素M1检测。分析采用市售的直接竞争酶联免疫吸附测定（ELISA）测试系统进行。样本用水（1:8）复溶，以1630×g离心15分钟，无需净化程序直接进行检测。结果显示，33份（11%）样本的黄曲霉毒素M1呈阳性，含量为0.10 - 1.00 ng/ml（平均值：0.27 +/- 0.20 ng/ml）。根据4个月大儿童（摄入量最高）的牛奶消费模式数据，估计平均每日摄入量为3.7 ng/kg体重/天。讨论了这些数据对人类健康的影响。
