Karabin G D, Farley M, Hallick R B
Nucleic Acids Res. 1984 Jul 25;12(14):5801-12. doi: 10.1093/nar/12.14.5801.
The gene for the Mr 32000 herbicide binding polypeptide of photosystem II has previously been mapped to the 5 kbp EcoRI fragment Eco I of Euglena gracilis chloroplast DNA. The nucleotide sequence of 3324 bp of Eco I, containing the psbA locus, has been determined. This locus encodes a polypeptide of 345 amino acids which is co-linear with, and has 86% derived amino acid sequence homology to sequences derived from four higher plants chloroplast psbA loci. The Euglena psbA gene contains four introns of size 435, 443, 434, and 617 bp. The four introns have conserved boundary sequences of the type previously described in the Euglena chloroplast gene (rbcL) for the large subunit of ribulose-1,5-bisphosphate carboxylase (Koller et al., Cell 36, 545-553, 1984).
光系统II中分子量为32000的除草剂结合多肽的基因先前已被定位到纤细裸藻叶绿体DNA的5kbp EcoRI片段Eco I上。已确定了包含psbA基因座的Eco I的3324bp核苷酸序列。该基因座编码一个由345个氨基酸组成的多肽,它与来自四个高等植物叶绿体psbA基因座的序列共线性,并且其推导的氨基酸序列同源性为86%。纤细裸藻psbA基因包含四个大小分别为435、443、434和617bp的内含子。这四个内含子具有先前在纤细裸藻叶绿体基因(rbcL)中描述的用于1,5-二磷酸核酮糖羧化酶大亚基的保守边界序列(科勒等人,《细胞》36卷,545 - 553页,1984年)。