Kartalov Emil P, Zhong Jiang F, Scherer Axel, Quake Stephen R, Taylor Clive R, Anderson W French
Dept of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, Los Angeles 90033, USA.
Biotechniques. 2006 Jan;40(1):85-90. doi: 10.2144/000112071.
Here we describe the development of a high-throughput multi-antigen microfluidic fluorescence immunoassay system. A 100-chamber polydimethylsiloxane (PDMS) chip performs up to 5 tests for each of 10 samples. In this particular study system, the specificity of detection was demonstrated, and calibration curves were produced for C-reactive protein (CRP), prostate-specific antigen (PSA), ferritin, and vascular endothelial growth factor (VEGF). The measurements show sensitivity at and below clinically normal levels (with a signal-to-noise ratio >8 at as low as 10 pM antigen concentration). The chip uses 100 nL per sample for all tests. The developed system is an important step toward derivative immunoassay applications in scientific research and "point-of-care" testing in medicine.
在此，我们描述了一种高通量多抗原微流控荧光免疫分析系统的开发。一个具有100个腔室的聚二甲基硅氧烷（PDMS）芯片可对10个样本中的每一个进行多达5次检测。在这个特定的研究系统中，展示了检测的特异性，并生成了C反应蛋白（CRP）、前列腺特异性抗原（PSA）、铁蛋白和血管内皮生长因子（VEGF）的校准曲线。测量结果显示在临床正常水平及以下具有灵敏度（在低至10 pM抗原浓度时信噪比>8）。该芯片对所有检测每个样本使用100 nL。所开发的系统是朝着免疫分析衍生应用在科学研究和医学“即时检测”方面迈出的重要一步。
