Balzarini J, Schols D, Neyts J, Van Damme E, Peumans W, De Clercq E
Laboratory of Chemotherapy, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Belgium.
Antimicrob Agents Chemother. 1991 Mar;35(3):410-6. doi: 10.1128/AAC.35.3.410.
The alpha-(1-3)-D-mannose- and alpha-(1-6)-D-mannose-specific agglutinins (lectins) from Galanthus nivalis, Hippeastrum hybrid, Narcissus pseudonarcissus, and Listera ovata inhibited infection of MT-4 cells by human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) and simian immunodeficiency virus at concentrations comparable to the concentrations at which dextran sulfate (molecular weight, 5,000 [DS-5000]) inhibits these viruses (50% effective concentration, 0.2 to 0.6 microgram/ml). Unlike DS-5000, however, the plant lectins did not inhibit the replication of other enveloped viruses, except for human cytomegalovirus (50% effective concentration, 0.9 to 1.6 microgram/ml). The plant lectins suppressed syncytium formation between persistently HIV-1- or HIV-2-infected HUT-78 cells and uninfected MOLT-4 (clone 8) cells at concentrations that were 5- to 10-fold lower than that required for DS-5000. Unlike DS-5000, however, the plant lectins did not inhibit HIV-1 binding to CD4+ cells. Combination of the plant lectins with DS-5000 led to a potent synergistic inhibition of HIV-1-induced cytopathogenicity in MT-4 cells and syncytium formation between HIV-infected HUT-78 cells and MOLT-4 cells. Our data suggest that alpha-(1-3)-D- and alpha-(1-6)-D-mannose-specific plant lectins interfere with an event in the HIV replicative cycle that is subsequent to the attachment of the virions to the cells (i.e., the fusion process).
来自雪花莲、朱顶红、水仙和羊耳蒜的α-(1-3)-D-甘露糖特异性和α-(1-6)-D-甘露糖特异性凝集素（植物凝集素）在与硫酸葡聚糖（分子量5000 [DS-5000]）抑制这些病毒的浓度相当的浓度下（50%有效浓度为0.2至0.6微克/毫升），抑制了1型和2型人类免疫缺陷病毒（HIV-1和HIV-2）以及猿猴免疫缺陷病毒对MT-4细胞的感染。然而，与DS-5000不同的是，除了人类巨细胞病毒（50%有效浓度为0.9至1.6微克/毫升）外，这些植物凝集素并不抑制其他包膜病毒的复制。这些植物凝集素在比DS-5000所需浓度低5至10倍的浓度下，抑制了持续感染HIV-1或HIV-2的HUT-78细胞与未感染的MOLT-4（克隆8）细胞之间的合胞体形成。然而，与DS-5000不同的是，这些植物凝集素并不抑制HIV-1与CD4+细胞的结合。植物凝集素与DS-5000联合使用，可有效协同抑制HIV-1在MT-4细胞中诱导的细胞病变效应以及HIV感染的HUT-78细胞与MOLT-4细胞之间的合胞体形成。我们的数据表明，α-(1-3)-D-和α-(1-6)-D-甘露糖特异性植物凝集素干扰了HIV复制周期中病毒粒子附着于细胞之后的一个事件（即融合过程）。
