Jauregui H O, Gann K L
Department of Pathology, Rhode Island Hospital, Providence 02903.
J Cell Biochem. 1991 Apr;45(4):359-65. doi: 10.1002/jcb.240450409.
Technological advances in the separation and culture of mammalian hepatocytes have facilitated the use of these cells as the foundation for either hepatocyte transplantation or hepatocyte-seeded hollow fiber liver assist devices (LAD). To fully appreciate the practical applications of these tissue engineering solutions, it is necessary to understand the types of human liver failure as well as the corresponding animal models. The most immediate application of this type of technology is the treatment of hepatic encephalopathy (HE), an acute and highly fatal complication of fulminant hepatic failure. Although the pathogenesis of HE is unknown, failure of the detoxification function of the liver is accepted as playing an important role in this disorder. Consequently, the assaying and preservation of P450 activity in the grafted cells or in the LAD must be among the main targets of this research. This review explores the problems in hepatocyte transplantation and culture that deserve special consideration and emphasizes the conditions contributing to the in vitro maintenance of phenotypic expression of these cells.
哺乳动物肝细胞分离和培养技术的进步促进了这些细胞的应用，使其成为肝细胞移植或肝细胞接种中空纤维肝辅助装置（LAD）的基础。为了充分理解这些组织工程解决方案的实际应用，有必要了解人类肝衰竭的类型以及相应的动物模型。这类技术最直接的应用是治疗肝性脑病（HE），这是暴发性肝衰竭的一种急性且高度致命的并发症。尽管HE的发病机制尚不清楚，但肝脏解毒功能的衰竭被认为在这种疾病中起重要作用。因此，检测和保存移植细胞或LAD中细胞色素P450的活性必定是本研究的主要目标之一。本综述探讨了肝细胞移植和培养中值得特别关注的问题，并强调了有助于这些细胞在体外维持表型表达的条件。
