Krugman S, Friedman H, Lattimer C
N Engl J Med. 1975 May 29;292(22):1141-3. doi: 10.1056/NEJM197505292922201.
Hepatitis A antibody was detected by specific immune adherence and complement-fixation tests in a study involving 473 serum specimens from 20 patients who had viral hepatitis, Type A. In all 20 patients who had no detectable immune adherence antibody (less than 1:5) before onset of hepatitis high levels (greater than or equal to 1:1024) developed one to four weeks later, occasionally reaching peak levels exceeding greater than or equal to 1:81,920 several months thereafter. Five to 10 years later the immune adherence antibody levels ranged between 1:640 and 1:20,480. In general, the complement-fixation test was not as sensitive or as specific as the immune adherence test. These findings indicate that the immune adherence test should be a valuable tool for diagnosis, for epidemiologic surveys, for identification of susceptible and immune persons, for quantitative assays of gamma globulin and for identification of hepatitis A virus in attempts to propagate the virus in cell culture.
在一项涉及20例甲型病毒性肝炎患者的473份血清标本的研究中，通过特异性免疫黏附试验和补体结合试验检测甲型肝炎抗体。在所有20例肝炎发作前未检测到免疫黏附抗体（低于1:5）的患者中，高水平（大于或等于1:1024）在1至4周后出现，偶尔在数月后达到峰值水平超过大于或等于1:81,920。5至10年后，免疫黏附抗体水平在1:640至1:20,480之间。一般来说，补体结合试验不如免疫黏附试验敏感或特异。这些发现表明，免疫黏附试验对于诊断、流行病学调查、易感和免疫人群的识别、丙种球蛋白的定量测定以及在细胞培养中传播病毒时甲型肝炎病毒的鉴定应是一种有价值的工具。
