病毒抗原补体结合试验与免疫黏附血凝试验之间抗体反应性的差异。
Difference in antibody reactivity between complement fixation and immune adherence hemagglutination tests with virus antigens.
作者信息
Inouye S, Matsuno S, Kono R
出版信息
J Clin Microbiol. 1981 Sep;14(3):241-6. doi: 10.1128/jcm.14.3.241-246.1981.
Abstract
Complement-fixing (CF) immunoglobulin M antibody to infantile gastroenteritis virus (a rotavirus) did not show the reactivity of immune adherence hemagglutination (IAHA). Early immunoglobulin G CF antibody produced both in patients and in guinea pigs experimentally infected with Japanese encephalitis virus (a flavivirus) had weak reactivity in IAHA test. However, late antibody showed higher titers by IAHA than by CF. These results suggested that early antibodies with lower affinity are inefficient in the IAHA reaction. The implications of this study are: (i) a low ratio of IAHA/CF antibody titers in a serum suggests a recent rotavirus infection; (ii) the IAHA reaction is more type specific than the CF reaction for identifying the serotype of antigenically cross-reacting viruses with hyperimmune sera.
摘要
针对婴儿肠胃炎病毒（一种轮状病毒）的补体结合（CF）免疫球蛋白M抗体未表现出免疫黏附血凝（IAHA）反应性。在感染日本脑炎病毒（一种黄病毒）的患者及实验感染的豚鼠体内产生的早期免疫球蛋白G CF抗体，在IAHA试验中反应较弱。然而，后期抗体通过IAHA检测显示的效价比CF检测更高。这些结果表明，亲和力较低的早期抗体在IAHA反应中效率较低。本研究的意义在于：（i）血清中IAHA/CF抗体效价比例较低提示近期轮状病毒感染；（ii）在使用超免疫血清鉴定抗原交叉反应病毒血清型时，IAHA反应比CF反应更具型特异性。
相似文献
1
Difference in antibody reactivity between complement fixation and immune adherence hemagglutination tests with virus antigens.病毒抗原补体结合试验与免疫黏附血凝试验之间抗体反应性的差异。
J Clin Microbiol. 1981 Sep;14(3):241-6. doi: 10.1128/jcm.14.3.241-246.1981.
2
Assay of human rotavirus antibody by immune adherence hemagglutination with a cultivable human rotavirus as antigen.以可培养的人轮状病毒为抗原，通过免疫黏附血凝试验检测人轮状病毒抗体。
J Clin Microbiol. 1982 Jan;15(1):163-5. doi: 10.1128/jcm.15.1.163-165.1982.
3
Antigenic characterization of human and animal rotaviruses by immune adherence hemagglutination assay (IAHA): evidence for distinctness of IAHA and neutralization antigens.通过免疫粘附血凝试验（IAHA）对人和动物轮状病毒进行抗原特性分析：IAHA抗原与中和抗原不同的证据
Infect Immun. 1981 Aug;33(2):415-25. doi: 10.1128/iai.33.2.415-425.1981.
4
[Detection of antibody to varicella zoster virus by immune adherence hemagglutination].[免疫粘连血凝法检测水痘-带状疱疹病毒抗体]
Kansenshogaku Zasshi. 1993 Aug;67(8):730-5. doi: 10.11150/kansenshogakuzasshi1970.67.730.
5
Detection of varicella-zoster antibody by immune adherence hemagglutination test.免疫粘连血凝试验检测水痘-带状疱疹抗体。
Southeast Asian J Trop Med Public Health. 1984 Sep;15(3):342-7.
6
Identification of group A coxsackieviruses by immune adherence hemagglutination.通过免疫粘附血凝反应鉴定A组柯萨奇病毒
Jpn J Med Sci Biol. 1983 Aug;36(4):199-207. doi: 10.7883/yoken1952.36.199.
7
Antibody assays for varicella-zoster virus: comparison of enzyme immunoassay with neutralization, immune adherence hemagglutination, and complement fixation.水痘带状疱疹病毒抗体检测：酶免疫测定法与中和试验、免疫粘附血凝试验及补体结合试验的比较
J Clin Microbiol. 1978 Nov;8(5):545-52. doi: 10.1128/jcm.8.5.545-552.1978.
8
Immune adherence hemagglutination: alternative to complement-fixation serology.免疫黏附血凝反应：补体结合血清学的替代方法。
J Clin Microbiol. 1978 Mar;7(3):282-5. doi: 10.1128/jcm.7.3.282-285.1978.
9
Comparison of 4 serological tests--complement fixation, neutralization, fluorescent antibody to membrane antigen and immune adherence hemagglutination--for assay of antibody to varicella-zoster (V-Z) virus.用于检测水痘-带状疱疹（V-Z）病毒抗体的4种血清学检测方法的比较——补体结合试验、中和试验、膜抗原荧光抗体试验和免疫黏附血凝试验。
Biken J. 1979 Jun;22(2):55-60.
10
An immune adherence assay for discrimination between etiologic agents of hemorrhagic fever with renal syndrome.一种用于区分肾综合征出血热病原体的免疫黏附试验。
J Infect Dis. 1984 Jan;149(1):67-73. doi: 10.1093/infdis/149.1.67.
引用本文的文献
1
Rapid test for detection of rabies antibodies in human serum.用于检测人血清中狂犬病抗体的快速检测法。
J Clin Microbiol. 1983 Mar;17(3):481-4. doi: 10.1128/jcm.17.3.481-484.1983.
2
Immune adherence hemagglutination test for detection of antibody to respiratory syncytial virus. Brief report.检测呼吸道合胞病毒抗体的免疫黏附血凝试验。简要报告。
Arch Virol. 1984;82(1-2):95-100. doi: 10.1007/BF01309371.
3
Infectivity titration of hemorrhagic fever with renal syndrome virus: use of immune adherence hemagglutination for detection of virus growth.肾综合征出血热病毒的感染性滴定：利用免疫黏附血凝反应检测病毒生长
J Clin Microbiol. 1984 Sep;20(3):483-5. doi: 10.1128/jcm.20.3.483-485.1984.
4
Assay of human rotavirus antibody by immune adherence hemagglutination with a cultivable human rotavirus as antigen.以可培养的人轮状病毒为抗原，通过免疫黏附血凝试验检测人轮状病毒抗体。
J Clin Microbiol. 1982 Jan;15(1):163-5. doi: 10.1128/jcm.15.1.163-165.1982.
5
Isolation of human rotaviruses in primary cultures of monkey kidney cells.在猴肾细胞原代培养物中分离人轮状病毒。
J Clin Microbiol. 1982 Aug;16(2):387-90. doi: 10.1128/jcm.16.2.387-390.1982.
6
Changes in antibody avidity after virus infections: detection by an immunosorbent assay in which a mild protein-denaturing agent is employed.病毒感染后抗体亲和力的变化：采用温和蛋白质变性剂的免疫吸附测定法进行检测。
J Clin Microbiol. 1984 Sep;20(3):525-9. doi: 10.1128/jcm.20.3.525-529.1984.
7
Single-serum diagnosis of rubella by combined use of the hemagglutination inhibition and passive hemagglutination tests.联合使用血凝抑制试验和被动血凝试验进行风疹的单份血清诊断。
J Clin Microbiol. 1986 Feb;23(2):388-91. doi: 10.1128/jcm.23.2.388-391.1986.
8
Epidemiological studies of hemorrhagic fever with renal syndrome (HFRS) related virus infection among urban rats in Hokkaido, Japan.日本北海道城市鼠类中肾综合征出血热（HFRS）相关病毒感染的流行病学研究。
Arch Virol. 1986;88(3-4):231-40. doi: 10.1007/BF01310877.
本文引用的文献
1
THE NATURE OF ANTIBODIES FOLLOWING EXPERIMENTAL ARBOVIRUS INFECTION IN GUINEA PIGS.豚鼠实验性虫媒病毒感染后抗体的性质
J Immunol. 1965 Jan;94:1-11.
2
Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses.节肢动物传播病毒的血凝及血凝抑制技术。
Am J Trop Med Hyg. 1958 Sep;7(5):561-73. doi: 10.4269/ajtmh.1958.7.561.
3
Serodiagnosis of viral hepatitis A by a solid-phase radioimmunoassay specific for IgM antibodies.用针对IgM抗体的固相放射免疫分析法对甲型病毒性肝炎进行血清学诊断。
Scand J Infect Dis. 1981;13(1):5-9. doi: 10.1080/00365548.1981.11690359.
4
Cytomegalovirus complement-fixing IgM antibody.巨细胞病毒补体结合IgM抗体
J Med Virol. 1980;5(3):183-93. doi: 10.1002/jmv.1890050303.
5
Patterns of shedding of hepatitis A virus antigen in feces and of antibody responses in patients with naturally acquired type A hepatitis.自然获得性甲型肝炎患者粪便中甲型肝炎病毒抗原的排出模式及抗体反应
J Infect Dis. 1980 Nov;142(5):654-9. doi: 10.1093/infdis/142.5.654.
6
Epidemiological and serological study of hepatitis A virus outbreaks in France: a comparison between immunoadherence and radioimmunoassay.法国甲型肝炎病毒暴发的流行病学和血清学研究：免疫粘连法与放射免疫测定法的比较
J Hyg (Lond). 1981 Apr;86(2):155-62. doi: 10.1017/s0022172400068868.
7
Serotyping of dengue viruses by an immune adherence hemagglutination test.通过免疫黏附血凝试验对登革病毒进行血清分型。
Am J Trop Med Hyg. 1980 Nov;29(6):1389-93. doi: 10.4269/ajtmh.1980.29.1389.
8
Detection of Australia antigen by means of immune adherence haemagglutination test.用免疫粘连血凝试验检测澳大利亚抗原。
Vox Sang. 1971 Feb;20(2):178-81. doi: 10.1111/j.1423-0410.1971.tb00549.x.
9
Standardized viral hemagglutination and hemagglutination-inhibition tests. I. Standardization of erythrocyte suspensions.标准化病毒血凝和血凝抑制试验。I. 红细胞悬液的标准化。
Appl Microbiol. 1969 Nov;18(5):816-23. doi: 10.1128/am.18.5.816-823.1969.
10
Immune adherence hemagglutination test as a new sensitive method for titration of animal virus antigens and antibodies.免疫粘连血凝试验作为一种滴定动物病毒抗原和抗体的新的灵敏方法。
Jpn J Med Sci Biol. 1966 Jun;19(3):109-26. doi: 10.7883/yoken1952.19.109.
Zotero 插件