Inouye S, Matsuno S, Kono R
J Clin Microbiol. 1981 Sep;14(3):241-6. doi: 10.1128/jcm.14.3.241-246.1981.
Complement-fixing (CF) immunoglobulin M antibody to infantile gastroenteritis virus (a rotavirus) did not show the reactivity of immune adherence hemagglutination (IAHA). Early immunoglobulin G CF antibody produced both in patients and in guinea pigs experimentally infected with Japanese encephalitis virus (a flavivirus) had weak reactivity in IAHA test. However, late antibody showed higher titers by IAHA than by CF. These results suggested that early antibodies with lower affinity are inefficient in the IAHA reaction. The implications of this study are: (i) a low ratio of IAHA/CF antibody titers in a serum suggests a recent rotavirus infection; (ii) the IAHA reaction is more type specific than the CF reaction for identifying the serotype of antigenically cross-reacting viruses with hyperimmune sera.
针对婴儿肠胃炎病毒（一种轮状病毒）的补体结合（CF）免疫球蛋白M抗体未表现出免疫黏附血凝（IAHA）反应性。在感染日本脑炎病毒（一种黄病毒）的患者及实验感染的豚鼠体内产生的早期免疫球蛋白G CF抗体，在IAHA试验中反应较弱。然而，后期抗体通过IAHA检测显示的效价比CF检测更高。这些结果表明，亲和力较低的早期抗体在IAHA反应中效率较低。本研究的意义在于：（i）血清中IAHA/CF抗体效价比例较低提示近期轮状病毒感染；（ii）在使用超免疫血清鉴定抗原交叉反应病毒血清型时，IAHA反应比CF反应更具型特异性。
