Coudray Clotilde, Guitard Evelyne, Keyser-Tracqui Christine, Melhaoui Mohamed, Cherkaoui Mohamed, Larrouy Georges, Dugoujon Jean-Michel
Anthropology Center, UMR 8555 CNRS, 37 Allées Jules Guesde, 31073 Toulouse, France.
Forensic Sci Int. 2007 Mar 22;167(1):81-6. doi: 10.1016/j.forsciint.2006.01.003. Epub 2006 Feb 7.
The allele frequency distribution of 15 short tandem repeats (STR) loci contained in the AmpFlSTR Identifiler PCR Amplification Kit (Applied Biosystems), was determined in two Berber populations from Asni and Bouhria, in Central and Eastern Morocco, respectively. A total of 209 individuals were typed. No deviations from the Hardy-Weinberg equilibrium were observed for Asni at the 15 STRs loci whereas for the Bouhria samples, two loci (D5S818 and TH01) showed significant departures from Hardy-Weinberg expectations (after Bonferroni's correction). All loci are highly polymorphic and population differentiation tests showed that the Moroccan samples from Asni and Bouhria have significant differences in 4 out of 15 loci (D21S11, D7S820, D16S539 and TPOX). The aim of the study was to obtain accurate allele frequencies relevant for forensic applications. Comparative analyses between our population data and other population samples gathered from the literature are also presented.
在分别来自摩洛哥中部阿斯尼和东部布赫里亚的两个柏柏尔人群中，测定了AmpFlSTR Identifiler PCR扩增试剂盒（应用生物系统公司）中包含的15个短串联重复序列（STR）位点的等位基因频率分布。共对209名个体进行了分型。在15个STR位点上，阿斯尼人群未观察到偏离哈迪-温伯格平衡的情况；而对于布赫里亚样本，两个位点（D5S818和TH01）显示出与哈迪-温伯格预期存在显著偏差（经邦费罗尼校正后）。所有位点均具有高度多态性，群体分化测试表明，来自阿斯尼和布赫里亚的摩洛哥样本在15个位点中的4个位点（D21S11、D7S820、D16S539和TPOX）存在显著差异。该研究的目的是获得与法医应用相关的准确等位基因频率。还展示了我们的群体数据与从文献中收集的其他群体样本之间的比较分析。
