Kastner Sabine, Perreten Vincent, Bleuler Helen, Hugenschmidt Gabriel, Lacroix Christophe, Meile Leo
Laboratory of Food Biotechnology, Institute of Food Science and Nutrition, Swiss Federal Institute of Technology (ETH), ETH-Zentrum, CH-8092 Zurich, Switzerland.
Syst Appl Microbiol. 2006 Mar;29(2):145-55. doi: 10.1016/j.syapm.2005.07.009. Epub 2005 Aug 18.
A survey of starter and probiotic cultures was carried out to determine the current antibiotic resistance situation in microbial food additives in Switzerland. Two hundred isolates from 90 different sources were typed by molecular and other methods to belong to the genera Lactobacillus (74 samples), Staphylococcus (33 samples), Bifidobacterium (6 samples), Pediococcus (5 samples), or were categorized as lactococci or streptococci (82 samples). They were screened for phenotypic resistances to 20 antibiotics by the disk diffusion method. Twenty-seven isolates exhibiting resistances that are not an intrinsic feature of the respective genera were further analyzed by microarray hybridization as a tool to trace back phenotypic resistances to specific genetic determinants. Their presence was finally verified by PCR amplification or Southern hybridization. These studies resulted in the detection of the tetracycline resistance gene tet(K) in 5 Staphylococcus isolates used as meat starter cultures, the tetracycline resistance gene tet(W) in the probiotic cultures Bifidobacterium lactis DSM 10140 and Lactobacillus reuteri SD 2112 (residing on a plasmid), and the lincosamide resistance gene lnu(A) (formerly linA) in L. reuteri SD 2112.
开展了一项关于发酵剂和益生菌培养物的调查，以确定瑞士微生物食品添加剂目前的抗生素耐药情况。通过分子方法和其他方法对来自90个不同来源的200株分离株进行分型，结果表明它们分别属于乳杆菌属（74个样本）、葡萄球菌属（33个样本）、双歧杆菌属（6个样本）、片球菌属（5个样本），或者被归类为乳球菌或链球菌（82个样本）。采用纸片扩散法对它们进行20种抗生素的表型耐药性筛查。对27株表现出不属于各自属固有特征的耐药性的分离株，进一步通过微阵列杂交进行分析，以此作为追溯表型耐药性至特定基因决定因素的工具。最后通过PCR扩增或Southern杂交验证它们的存在。这些研究结果在用作肉类发酵剂培养物的5株葡萄球菌分离株中检测到四环素耐药基因tet(K)，在益生菌培养物乳酸双歧杆菌DSM 10140和罗伊氏乳杆菌SD 2112（存在于质粒上）中检测到四环素耐药基因tet(W)，以及在罗伊氏乳杆菌SD 2112中检测到林可酰胺耐药基因lnu(A)（原linA）。
