仓鼠抗α-鹅膏蕈碱RNA聚合酶II能够在体细胞杂种中转录多瘤病毒基因组。
Hamster alpha-amanitine-resistant RNA polymerase II able to transcribe polyoma virus genome in somatic cell hybrids.
作者信息
Amati P, Blasi F, Di Porzio U, Riccio A, Traboni C
出版信息
Proc Natl Acad Sci U S A. 1975 Feb;72(2):753-7. doi: 10.1073/pnas.72.2.753.
Abstract
A hamster cell line resistant to alpha-amanitine has been isolated (alpha-am-r, BHK-T6-G-1). Cell extracts of this mutant have an alpha-amanitine-resistant RNA polymerase II (nucleosidetriphosphate: RNA nucleotidyl-transferase, EC 2.7.7.6) activity as shown by DEAE-cellulose column chromatography. This mutation is dominant in interspecific hybrids with 3T3 mouse cells. In such hybrids polyoma virus can grow with equal efficiency in the presence or absence of the drug, thus indicating that the RNA polymerase of the unsusceptible parental cell can participate in the correct transcription of the viral genome.
摘要
已分离出一种对α-鹅膏蕈碱具有抗性的仓鼠细胞系（α-am-r，BHK-T6-G-1）。如通过二乙氨基乙基纤维素柱色谱法所示，该突变体细胞提取物具有对α-鹅膏蕈碱抗性的RNA聚合酶II（核苷三磷酸：RNA核苷酸转移酶，EC 2.7.7.6）活性。这种突变在与3T3小鼠细胞的种间杂种中是显性的。在这种杂种中，多瘤病毒在有或没有该药物的情况下都能以相同效率生长，因此表明不敏感亲代细胞的RNA聚合酶可以参与病毒基因组的正确转录。
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