Severinová J, Salát J, Krocová Z, Reznícková J, Demová H, Horká H, Kopecký J
Institute of Parasitology, Academy of Sciences of the Czech Republic and Faculty of Biological Sciences, University of South Bohemia, 370 05 Ceské Budĕjovice, Czechia.
Folia Microbiol (Praha). 2005;50(5):457-63. doi: 10.1007/BF02931430.
The impact of Ixodes ricinus salivary gland extract (SGE) on inflammatory changes in the skin and draining lymph nodes of mice, elicited by the infection with the important human pathogen, B. afzelii, was determined using flow cytometry. SGE injected together with spirochetes reduced the numbers of leukocytes and gammadelta-T lymphocytes in infected epidermis at early time-points post infection. In draining lymph nodes, the anti-inflammatory effect of SGE was manifested by the decrease of total cell count compared with that in mice treated with inactivated SGE. Changes in subpopulations of immunocompetent cells apparently reflected the effect of SGE on the proliferation of spirochetes in the host. The significance of tick saliva anti-inflammatory effect for saliva activated transmission of B. afzelii is shown.
利用流式细胞术确定了蓖麻硬蜱唾液腺提取物（SGE）对由重要人类病原体阿氏疏螺旋体感染引起的小鼠皮肤和引流淋巴结炎症变化的影响。与螺旋体一起注射的SGE在感染后早期减少了感染表皮中白细胞和γδ-T淋巴细胞的数量。在引流淋巴结中，与用灭活SGE处理的小鼠相比，SGE的抗炎作用表现为总细胞数减少。免疫活性细胞亚群的变化显然反映了SGE对宿主中螺旋体增殖的影响。研究显示了蜱唾液抗炎作用对阿氏疏螺旋体唾液激活传播的重要性。
