Lacroix Magali, Léonil Joëlle, Bos Cécile, Henry Gwénaëlle, Airinei Gheorge, Fauquant Jacques, Tomé Daniel, Gaudichon Claire
Unit of Nutrition Physiology and Feeding Control, National Institute for Agricultural Research, UMR 914, National Institute for Agricultural Sciences of Paris, 16 rue Claude Bernard, 75231, Paris, France.
J Agric Food Chem. 2006 Feb 22;54(4):1508-17. doi: 10.1021/jf051304d.
To determine the bioavailability of industrially heat-treated milk proteins, male Wistar rats were given [15N]-labeled meals containing either nonheated-micellar casein (CAS), milk soluble protein isolate (MSPI), and microfiltered milk (MF)-or heated products-"high temperature short time" pasteurized (HTST), "higher temperature, shorter time" pasteurized (HHST), ultrahigh temperature-treated (UHT), and spray-dried (SPRAY) milks. The postprandial distribution of dietary nitrogen was measured in the splanchnic area and urea. Digestibility was around 96% except for SPRAY (94%) and MSPI (98%). Ingested nitrogen recovered in the splanchnic bed was 19.3% for SPRAY, 16.7% for MF, and around 14-15% for other products. Deamination of dietary nitrogen reached 21.2, 20.6, and 18.2% of ingested nitrogen for MSPI, SPRAY, and CAS, respectively, and around 14-16% for other products. In our model, only spray drying led to a significant increase of splanchnic extraction. Moreover, the biological value of purified protein fractions appeared to be lower than that seen in products containing total milk protein.
为了确定工业热处理乳蛋白的生物利用度，给雄性Wistar大鼠喂食含有未加热的胶粒酪蛋白（CAS）、乳清分离蛋白（MSPI）和微滤乳（MF）的[15N]标记膳食，或喂食加热产品——“高温短时”巴氏杀菌乳（HTST）、“更高温度、更短时间”巴氏杀菌乳（HHST）、超高温处理乳（UHT）和喷雾干燥乳（SPRAY）。测定了餐后膳食氮在内脏区域和尿素中的分布。除了喷雾干燥乳（94%）和乳清分离蛋白（98%）外，消化率约为96%。喷雾干燥乳在内脏床中回收的摄入氮为19.3%，微滤乳为16.7%，其他产品约为14 - 15%。膳食氮的脱氨基作用分别占乳清分离蛋白、喷雾干燥乳和胶粒酪蛋白摄入氮的21.2%、20.6%和18.2%，其他产品约为14 - 16%。在我们的模型中，只有喷雾干燥导致内脏提取显著增加。此外，纯化蛋白组分的生物学价值似乎低于含有全乳蛋白的产品。
