Schoenbaum M A, Freund J D, Beran G W
Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames 50011.
J Am Vet Med Assoc. 1991 Apr 15;198(8):1393-7.
Because of the importance of environmental survival of pseudorabies virus to proposals to eradicate the virus from swine in the United States, survival of the virus was studied in various diluents and on combinations of diluents and solid fomites at 25 C. Suspensions of the virus in phosphate-buffered saline and saline G solutions remained infectious for at least 10 days. Infectivity of other virus/diluent suspensions decreased to less than 10 plaque-forming units/ml in 14 days (swine urine), 7 days (well water), 4 days (swine saliva), 2 days (lagoon water and swine nasal washings), and 1 day (swine pit effluent, chlorinated water, and bile). Suspensions of pseudorabies virus in saline G solution and on the solid fomites, whole corn, and steel remained infectious for at least 7 days. Infectivity of other virus/diluent/fomite combinations decreased to less than 10 plaque-forming units/ml in 7 days. The role of the fomites as vehicles for transmission of infection is discussed.
鉴于伪狂犬病病毒的环境生存能力对于美国从猪群中根除该病毒的提议至关重要，因此研究了该病毒在25摄氏度下于各种稀释剂以及稀释剂与固体污染物组合中的生存情况。病毒在磷酸盐缓冲盐水和生理盐水G溶液中的悬液至少10天仍具感染性。其他病毒/稀释剂悬液的感染性在14天内（猪尿液）、7天内（井水）、4天内（猪唾液）、2天内（泻湖水和猪鼻洗液）以及1天内（猪粪池废水、氯化水和胆汁）降至低于10个空斑形成单位/毫升。伪狂犬病病毒在生理盐水G溶液中以及在固体污染物、整粒玉米和钢铁上的悬液至少7天仍具感染性。其他病毒/稀释剂/污染物组合的感染性在7天内降至低于10个空斑形成单位/毫升。文中讨论了污染物作为感染传播媒介的作用。
