Pirtle E C, Sacks J M, Nachman R J
Am J Vet Res. 1986 Sep;47(9):1892-5.
Butylated hydroxytoluene (BHT) was evaluated for antiviral effectiveness on pseudorabies virus (PRV) in cell culture, mice, and swine. When relatively small amounts of BHT were mixed with PRV and incubated at 37 C for 30 or 60 minutes before inoculation into cell cultures, the cell cultures did not become infected with virus. The PRV was not infectious when the virus was treated with BHT and then inoculated intraperitoneally into mice, but was infectious when BHT and PRV were inoculated simultaneously or when BHT was inoculated either 30 or 60 minutes before PRV. Swine fed BHT-medicated feed for 10 days before they were intranasally exposed with virulent PRV did not have overt signs of pseudorabies, had a lower concentration of PRV in nasal mucus than did control swine, and had acceptable blood enzyme and cholesterol concentrations during the experiment. The BHT was detected in tissues of 2 swine after they were fed BHT-medicated feed for 10 days, and higher concentrations of BHT were detected in tissues of 3 swine given BHT feed for 29 days.
对叔丁基苯酚（BHT）在细胞培养、小鼠和猪体内针对伪狂犬病病毒（PRV）的抗病毒效果进行了评估。当在接种到细胞培养物之前，将相对少量的BHT与PRV混合并在37℃下孵育30或60分钟时，细胞培养物未被病毒感染。用BHT处理病毒后经腹腔接种到小鼠体内时，PRV没有传染性，但当BHT和PRV同时接种或BHT在PRV接种前30或60分钟接种时，PRV具有传染性。在经鼻暴露于强毒PRV之前，用含BHT的饲料喂养猪10天，猪没有出现伪狂犬病的明显症状，鼻黏液中PRV的浓度低于对照猪，并且在实验期间血液酶和胆固醇浓度正常。在2头猪食用含BHT的饲料10天后，在其组织中检测到了BHT，在3头猪食用含BHT的饲料29天后，在其组织中检测到了更高浓度的BHT。
