Jones A, Morton I, Hobson L, Evans G S, Everard M L
Health and Safety Laboratory, Buxton, UK.
Clin Exp Immunol. 2006 Mar;143(3):513-22. doi: 10.1111/j.1365-2249.2005.03004.x.
RSV causes annual epidemics of bronchiolitis in winter months resulting in the hospitalization of many infants and the elderly. Dendritic cells (DCs) play a pivotal role in coordinating immune responses to infection and some viruses skew, or subvert, the immune functions of DCs. RSV infection of DCs could alter their function and this could explain why protection after natural RSV infection is incomplete and of short duration. In this study, this interaction between DCs and RSV was investigated using a human primary culture model. DCs were generated from purified healthy adult volunteer peripheral blood monocytes. Effects of RSV upon DC phenotype with RSV primed DCs was measured using flow cytometry. Changes to viability and proliferation of cocultured DCs and T-cells were determined using microscopy with fluorescent dyes (Hoechst 33342 and propidium iodide). DC maturation was not prevented by the RSV challenge. RSV infected a fraction of DCs (10-30%) but the virus replicated slowly in these cells with only small reduction to cell viability. DCs challenged with RSV stimulated T-cell proliferation less well than lipopolysaccharide. This is the first study to demonstrate RSV infection of human monocyte derived DCs and suggests that the virus does not significantly interfere with the function of these cells and potentially may promote cellular rather than humoral responses.
呼吸道合胞病毒（RSV）在冬季引发每年一度的细支气管炎流行，导致许多婴儿和老年人住院。树突状细胞（DCs）在协调针对感染的免疫反应中起关键作用，一些病毒会扭曲或破坏DCs的免疫功能。DCs感染RSV可能会改变其功能，这可以解释为什么自然感染RSV后的保护作用不完整且持续时间短。在本研究中，使用人类原代培养模型研究了DCs与RSV之间的这种相互作用。DCs由纯化的健康成年志愿者外周血单核细胞生成。使用流式细胞术测量RSV对经RSV预处理的DCs表型的影响。使用荧光染料（Hoechst 33342和碘化丙啶）通过显微镜检查确定共培养的DCs和T细胞的活力和增殖变化。RSV攻击并未阻止DC成熟。RSV感染了一部分DCs（10 - 30%），但病毒在这些细胞中复制缓慢，对细胞活力仅有轻微降低。用RSV攻击的DCs刺激T细胞增殖的效果不如脂多糖。这是第一项证明人类单核细胞衍生的DCs感染RSV的研究，表明该病毒不会显著干扰这些细胞的功能，并且可能促进细胞免疫反应而非体液免疫反应。
