Hollmann F, Hofstetter K, Schmid A
Degussa Care & Surface Specialties, Goldschmidt AG, Goldschmidtstrasse 100, 45127 Essen, Germany.
Trends Biotechnol. 2006 Apr;24(4):163-71. doi: 10.1016/j.tibtech.2006.02.003. Epub 2006 Feb 20.
Biocatalytic oxygenation chemistry is a rapidly evolving field in which monooxygenases are the tools of choice. Monooxygenases catalyze many industrially important synthetic transformations; however, their use in preparative applications is hampered by their intrinsic requirement for reducing equivalents. As a result, non-enzymatic strategies--where the reducing equivalents are introduced directly into the catalytic cycle--are being developed to supersede the well-established enzymatic NAD(P)H regeneration systems currently in use. In this review we summarize and evaluate recent achievements in this area.
生物催化氧化化学是一个快速发展的领域，其中单加氧酶是首选工具。单加氧酶催化许多具有重要工业意义的合成转化反应；然而，它们在制备应用中的使用受到其对还原当量的内在需求的阻碍。因此，正在开发非酶策略——将还原当量直接引入催化循环——以取代目前使用的成熟的酶促NAD(P)H再生系统。在这篇综述中，我们总结并评估了该领域的最新成就。
