Zhang Cui
Harbin University of Commerce, College of Medicine, Harbin 150076, China.
Zhongguo Zhong Yao Za Zhi. 2005 Dec;30(24):1953-6.
To study on the regulation of leucocyte adhesion molecule CD11b, CD44 (CD11b, CD44) induced by Qichou decoction in the rats with kidney cut off largely.
The animal model was established by excising five sixth of kidney bilaterally. The rats were randomly divided into sham-operation group, model group with kidney cut off largely, the group treated by aldehyde oxygen amylum, the group treated with Shenshuaining and groups treated by high, moderate and low doses of Qichou decoction. The animals in the sham-operation group and model group were given with equated physiological brine. The dosages of aldehyde oxygen amylum and Shenshuaining were 0.9 g x kg(-1) and 0.37 g x kg(-1) respectively. The high, moderate and low dosages of Qichou decoction were 53.1, 26.6 g x kg(-1) and 13.3 g x kg(-1) respectively. The contents of leucocyte adhesion molecules including CD11b and CD44 in microserum were labeled by fluorescein and measured by flow Cytometry. Tumor necrosis factor (TNF-alpha)was detected by Dot-ELISA assay.
The expression of CD11b, CD44 and obviously in the model group as compared with sham-operation group. The weights of left kidney, the ratio of left kidney TNF-alpha was increased weight/body weight. BUN and Scr were also increased obviously. After the treatment with Qichou decoction, the contents of CD11b, CD44, TNF-a, BUN and Scr in the rats with kidney cut off largely were reduced significantly. Similarly, the weight of left kidney and the ratio of the left kidney weight/body weight were also decreased obviously.
Leucocyte adhesion molecules including CD11b and CD44 in the rats with largely partial nephrectomy were significantly regulated by Qichou decoction. Qichou decoction can prevent immersion of the cells and inflammation, inhibit compensatory glomerular hypertrophy of the rest kidney, prevent the glomerular interstitial fibrosis, and protect the kidney in the rats with kidney cut off largely from further impairment.
研究芪丑汤对大部分肾切除大鼠白细胞黏附分子CD11b、CD44（CD11b、CD44）的调控作用。
采用双侧切除五分之六肾脏建立动物模型。将大鼠随机分为假手术组、大部分肾切除模型组、醛氧淀粉治疗组、肾衰宁治疗组以及芪丑汤高、中、低剂量治疗组。假手术组和模型组给予等量生理盐水。醛氧淀粉和肾衰宁的剂量分别为0.9 g·kg⁻¹和0.37 g·kg⁻¹。芪丑汤高、中、低剂量分别为53.1、26.6 g·kg⁻¹和13.3 g·kg⁻¹。用荧光素标记微量血清中包括CD11b和CD44在内的白细胞黏附分子含量，采用流式细胞术进行检测。采用斑点酶联免疫吸附测定法检测肿瘤坏死因子（TNF-α）。
与假手术组相比，模型组CD11b、CD44表达明显增加。左肾重量、左肾重量/体重比值以及TNF-α均升高。血尿素氮（BUN）和血肌酐（Scr）也明显升高。芪丑汤治疗后，大部分肾切除大鼠的CD11b、CD44、TNF-α、BUN和Scr含量显著降低。同样，左肾重量和左肾重量/体重比值也明显降低。
芪丑汤对大部分肾切除大鼠的白细胞黏附分子CD11b和CD44有显著调控作用。芪丑汤可防止细胞浸润和炎症，抑制剩余肾脏的代偿性肾小球肥大，预防肾小球间质纤维化，保护大部分肾切除大鼠的肾脏免受进一步损害。
