Ceelen Liesbeth M, Decostere Annemie, Van den Bulck Kathleen, On Stephen L W, Baele Margo, Ducatelle Richard, Haesebrouck Freddy
Ghent University, Merelbeke, Belgium.
Emerg Infect Dis. 2006 Feb;12(2):263-7. doi: 10.3201/eid1202.050847.
A total of 110 broilers from 11 flocks were tested for Helicobacter pullorum by polymerase chain reaction; positive samples were reexamined with a conventional isolation method. H. pullorum isolates were examined by amplified fragment length polymorphism (AFLP) fingerprinting for interstrain genetic diversity and relatedness. Sixteen isolates from cecal samples from 2 different flocks were obtained. AFLP analysis showed that these isolates and 4 additional isolates from a different flock clustered according to their origin, which indicates that H. pullorum colonization may occur with a single strain that disseminates throughout the flock. Strains isolated from different hosts or geographic sources displayed a distinctive pattern. H. pullorum is present in approximately one third of live chickens in Belgium and may represent a risk to human health.
通过聚合酶链反应对来自11个鸡群的总共110只肉鸡进行了鸡幽门螺杆菌检测；对阳性样本采用传统分离方法进行复检。通过扩增片段长度多态性（AFLP）指纹分析对鸡幽门螺杆菌分离株进行菌株间遗传多样性和相关性检测。从2个不同鸡群的盲肠样本中获得了16株分离株。AFLP分析表明，这些分离株以及来自另一个鸡群的另外4株分离株按其来源聚类，这表明鸡幽门螺杆菌的定植可能是由单一菌株传播至整个鸡群所致。从不同宿主或地理来源分离出的菌株呈现出独特的模式。在比利时，约三分之一的活鸡中存在鸡幽门螺杆菌，这可能对人类健康构成风险。
