Carbone Roberta, Marangi Ida, Zanardi Andrea, Giorgetti Luca, Chierici Elisabetta, Berlanda Giuseppe, Podestà Alessandro, Fiorentini Francesca, Bongiorno Gero, Piseri Paolo, Pelicci Pier Giuseppe, Milani Paolo
European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy.
Biomaterials. 2006 Jun;27(17):3221-9. doi: 10.1016/j.biomaterials.2006.01.056. Epub 2006 Feb 28.
We have characterized the biocompatibility of nanostructured TiO2 films produced by the deposition of a supersonic beam of TiOx clusters. Physical analysis shows that these films possess, at the nanoscale, a granularity and porosity mimicking those of typical extracellular matrix structures and adsorption properties that could allow surface functionalization with different macromolecules such as DNA, proteins, and peptides. To explore the biocompatibility of this novel nanostructured surface, different cancer and primary cells were analyzed in terms of morphological appearance (by bright field microscopy and immunofluorescence) and growth properties, with the aim to evaluate cluster-assembled TiO2 films as substrates for cell-based and tissue-based applications. Our results strongly suggest that this new biomaterial supports normal growth and adhesion of primary and cancer cells with no need for coating with ECM proteins; we thus propose this new material as an optimal substrate for different applications in cell-based assays, biosensors or microfabricated medical devices.
我们已经对通过沉积TiOx团簇的超音速束流制备的纳米结构TiO2薄膜的生物相容性进行了表征。物理分析表明，这些薄膜在纳米尺度上具有类似于典型细胞外基质结构的粒度和孔隙率，以及吸附特性，这使得它们能够用不同的大分子（如DNA、蛋白质和肽）进行表面功能化。为了探索这种新型纳米结构表面的生物相容性，我们通过明场显微镜和免疫荧光分析了不同癌细胞和原代细胞的形态外观，并研究了其生长特性，目的是评估团簇组装TiO2薄膜作为基于细胞和组织应用的基质。我们的结果有力地表明，这种新型生物材料能够支持原代细胞和癌细胞的正常生长和黏附，无需用细胞外基质蛋白进行包被；因此，我们建议将这种新材料作为基于细胞的检测、生物传感器或微制造医疗设备等不同应用的理想基质。
