Jang Young-Su, Jeon Ju-Hyun, Lee Hoi-Seon
Faculty of Biotechnology and Research Center for Industrial Development of Biofood Materials, College of Agriculture and Life Science, Chonbuk National University, Chonju, South Korea.
J Am Mosq Control Assoc. 2005 Dec;21(4):400-3. doi: 10.2987/8756-971X(2006)21[400:MLAOAC]2.0.CO;2.
Mosqutio larvicidal activity of Chamaecyparis obtusa leaf-derived materials against the 4th-stage larvae of Aedes aegypti (L.), Ochlerotatus togoi (Theobald), and Culex pipiens pallens (Coquillett) was examined in the laboratory. A crude methanol extract of C. obtusa leaves was found to be active (percent mortality rough) against the 3 species larvae; the hexane fraction of the methanol extract showed a strong larvicidal activity (100% mortality) at 100 ppm. The bioactive component in the C. obtusa leaf extract was characterized as beta-thujaplicin by spectroscopic analyses. The LC50 value of beta-thujaplicin was 2.91, 2.60, and 1.33 ppm against Ae. aegypti, Oc. togoi, and Cx. pipiens pallens larvae. This naturally occurring C. obtusa leaves-derived compound merits further study as a potential mosquito larval control agent or lead compound.
在实验室中检测了钝叶扁柏叶提取物对埃及伊蚊、东乡伊蚊和淡色库蚊四龄幼虫的杀蚊幼虫活性。发现钝叶扁柏叶的粗甲醇提取物对这三种幼虫具有活性（粗略死亡率）；甲醇提取物的己烷馏分在100 ppm时表现出很强的杀幼虫活性（100%死亡率）。通过光谱分析将钝叶扁柏叶提取物中的生物活性成分鉴定为β-崖柏素。β-崖柏素对埃及伊蚊、东乡伊蚊和淡色库蚊幼虫的LC50值分别为2.91、2.60和1.33 ppm。这种天然存在的钝叶扁柏叶衍生化合物作为一种潜在的杀蚊幼虫剂或先导化合物值得进一步研究。
