Sawant Rupa, Torchilin Vladimir
Research Associate Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA, USA.
Methods Mol Biol. 2011;683:431-51. doi: 10.1007/978-1-60761-919-2_31.
Cell-penetrating peptides (CPPs), in particular TATp, have been widely used for intracellular delivery of various cargoes, both in vitro and in vivo. Modifications of nanoparticles with CPPs require either covalent or noncovalent approach. Here we describe various methods to attach CPP, such as TATp to surface of nanocarriers (such as liposomes and micelles), loading with drug or DNA and characterization of same for in vitro and in vivo applications. Due to nonselectivity of CPPs and wide distribution in nontarget areas, method for preparation of "smart" nanocarrier with hidden TATp function is also described.
细胞穿透肽（CPPs），尤其是TATp，已在体外和体内广泛用于各种货物的细胞内递送。用CPPs修饰纳米颗粒需要共价或非共价方法。在这里，我们描述了将CPP（如TATp）连接到纳米载体（如脂质体和胶束）表面的各种方法，装载药物或DNA以及对其进行体外和体内应用表征。由于CPPs的非选择性以及在非靶区域的广泛分布，还描述了制备具有隐藏TATp功能的“智能”纳米载体的方法。
