Aouad G, Crovisier J-L, Geoffroy V A, Meyer J-M, Stille P
Ecole et Observatoire des Sciences de la Terre, Centre de Géochimie de la Surface/CNRS UMR 7517, 1 rue Blessig, 67084 Strasbourg Cedex, France.
J Hazard Mater. 2006 Aug 25;136(3):889-95. doi: 10.1016/j.jhazmat.2006.01.026. Epub 2006 Feb 28.
A basaltic glass and a vitrified bottom ash were incubated at 25 degrees C in a growth medium (based on casaminoacids) inoculated with Pseudomonas aeruginosa. Bacterial growth and mineral concentrations in different compartments (bacterial cells, growth medium and biofilm) were monitored in short-term (3 days), and long-term experiments involving repeated renewals of the culture medium during 174 days. In short-term experiments, while the concentration of iron increased in the presence of bacteria, a decrease in Ni and Zn was observed in the growth medium compared to the sterile condition. During long-term experiments, such differences gradually decreased and disappeared after 78 days. On the contrary, iron concentration remained higher in the biotic condition compared to the sterile one. Bacterial growth resulted within a few days in the formation of a biofilm, which lead to the cementation of the altered glass grains. Most of the constituents of the glass (Si, Mg, Fe, Ti, Ba, Co, Zn, Cu, Ni and Cr) were found in the biofilm, while the chemical composition of the bacterial cells was very different.
将玄武岩玻璃和玻璃化底灰在25摄氏度下于接种了铜绿假单胞菌的生长培养基（基于酪蛋白氨基酸）中培养。在短期实验（3天）以及涉及在174天内反复更换培养基的长期实验中，监测不同隔室（细菌细胞、生长培养基和生物膜）中的细菌生长和矿物质浓度。在短期实验中，虽然在有细菌存在的情况下铁的浓度增加，但与无菌条件相比，生长培养基中的镍和锌含量下降。在长期实验中，这种差异逐渐减小，并在78天后消失。相反，与无菌条件相比，生物条件下的铁浓度仍然较高。细菌生长在几天内导致生物膜形成，这导致了蚀变玻璃颗粒的胶结。玻璃的大多数成分（硅、镁、铁、钛、钡、钴、锌、铜、镍和铬）都在生物膜中被发现，而细菌细胞的化学成分则非常不同。
