Graham A K, Herrington C S, McGee J O
University of Oxford, Nuffeld Department of Pathology and Bacteriology, John Radcliffe Hospital, Headington.
J Clin Pathol. 1991 Feb;44(2):96-101. doi: 10.1136/jcp.44.2.96.
The sensitivity and specificity of immunocytochemistry were compared with those of non-isotopic in situ hybridisation (NISH) for the direct detection of human papillomaviruses in biopsy specimens. Four monoclonal antibodies raised to the capsid protein of HPV16 were less specific than NISH: all four reacted with lesions containing HPV33, and HPV18. Absolute discrimination of HPV types, therefore, was not possible with the monoclonal antibodies used in this study. The relative sensitivities of these antibodies were also lower than NISH. Sequential immunocytochemistry and NISH on the same section showed that 2.9-13.0 times as many cells were positive by NISH than by immunocytochemistry using the most sensitive monoclonal antibody. These data indicate that NISH has higher diagnostic specificity and sensitivity than immunocytochemistry using monoclonal antibodies to the HPV16 capsid protein.
为了在活检标本中直接检测人乳头瘤病毒，对免疫细胞化学与非同位素原位杂交（NISH）的敏感性和特异性进行了比较。针对人乳头瘤病毒16型（HPV16）衣壳蛋白产生的四种单克隆抗体，其特异性低于NISH：所有四种抗体均与含有HPV33和HPV18的病变发生反应。因此，使用本研究中的单克隆抗体无法对HPV类型进行绝对区分。这些抗体的相对敏感性也低于NISH。在同一切片上进行连续免疫细胞化学和NISH检测表明，NISH检测出的阳性细胞数量是使用最敏感单克隆抗体进行免疫细胞化学检测的2.9至13.0倍。这些数据表明，与使用针对HPV16衣壳蛋白的单克隆抗体进行免疫细胞化学相比，NISH具有更高的诊断特异性和敏感性。
