Paris S, Meyer-Lueckel H, Mueller J, Hummel M, Kielbassa A M
Department of Operative Dentistry and Periodontology, University School of Dental Medicine, Berlin, Germany.
Caries Res. 2006;40(2):124-9. doi: 10.1159/000091058.
The use of sealants for the infiltration of proximal enamel lesions could be a promising alternative to the common strategies of remineralization and operative treatment. The aim of the present study was to compare the progression of sealed initial enamel lesions after exposure to a demineralizing solution in vitro. In each of 54 bovine enamel specimens three subsurface lesions were created. Two of the lesions were etched with phosphoric acid and sealed with either a fissure sealant or with various adhesives (1-5) for 15 s or 30 s, respectively, whereas one lesion remained as the untreated control. Subsequently, half of each specimen was covered with nail varnish (baseline) and the other half was reexposed to a demineralizing solution for 14 days (experimental). The specimens were cut perpendicularly to the surface, infiltrated with a low-viscosity fluorescent resin and observed with a confocal laser scanning microscope (CLSM). For lesions sealed with the fissure sealant and adhesives 1-3, the progression of lesion depth (0-31 microm) was significantly decreased (p<0.01; paired t test) compared with the untreated control (57 microm). For the fissure sealant and adhesives 1 and 3 extended penetration times (30 s) resulted in significantly reduced lesion progression compared to 15 s. It can be concluded that filling of the pores in initial enamel lesions with the fissure sealant and adhesives 1-3 can inhibit further demineralization in vitro.
使用封闭剂渗入近端釉质病变可能是再矿化和手术治疗等常见策略的一种有前景的替代方法。本研究的目的是比较体外暴露于脱矿溶液后封闭的初始釉质病变的进展情况。在54个牛牙釉质标本中，每个标本制造三个亚表面病变。其中两个病变用磷酸蚀刻，分别用窝沟封闭剂或各种粘合剂（1 - 5）封闭15秒或30秒，而一个病变作为未处理的对照。随后，每个标本的一半用指甲油覆盖（基线），另一半重新暴露于脱矿溶液中14天（实验）。将标本垂直于表面切割，用低粘度荧光树脂渗透，并用共聚焦激光扫描显微镜（CLSM）观察。对于用窝沟封闭剂和粘合剂1 - 3封闭的病变，与未处理的对照（57微米）相比，病变深度（0 - 31微米）的进展显著降低（p<0.01；配对t检验）。对于窝沟封闭剂和粘合剂1和3，与15秒相比，延长渗透时间（30秒）导致病变进展显著降低。可以得出结论，用窝沟封闭剂和粘合剂1 - 3填充初始釉质病变中的孔隙可以在体外抑制进一步脱矿。
