Brown I H, Paton D J
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey.
Vet Rec. 1991 May 25;128(21):500-3. doi: 10.1136/vr.128.21.500.
A competitive ELISA which differentiates between transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) was used to detect non-neutralising antibodies to the peplomer protein of TGEV in porcine sera. The test was shown to be TGEV specific, having a relative specificity of 100 per cent, and to have a relative sensitivity of 94.9 per cent when compared with the virus neutralisation test. The prevalence of TGEV in Great Britain is low; only 0.6 per cent of sows sampled in 1990 were seropositive to TGEV. Seroconversion to the virus neutralisation test occurred in a closed herd in 1984, with no apparent spread, but later testing by the ELISA did not detect any blocking antibodies. The possibility of the existence of a less contagious strain of PRCV is discussed. All British isolates of TGEV tested by the indirect fluorescent antibody test were recognised by the monoclonal antibody 1D.B12, the indicator antibody in the ELISA.
采用一种能区分传染性胃肠炎病毒（TGEV）和猪呼吸道冠状病毒（PRCV）的竞争性酶联免疫吸附测定（ELISA），检测猪血清中针对TGEV纤突蛋白的非中和抗体。结果表明，该检测方法具有TGEV特异性，相对特异性为100%，与病毒中和试验相比，相对敏感性为94.9%。英国TGEV的流行率较低；1990年采样的母猪中只有0.6%对TGEV血清呈阳性。1984年，一个封闭猪群中出现了针对病毒中和试验的血清转化，且无明显传播，但后来通过ELISA检测未发现任何阻断抗体。文中讨论了存在传染性较低的PRCV毒株的可能性。通过间接荧光抗体试验检测的所有英国TGEV分离株，均能被ELISA中的指示抗体单克隆抗体1D.B12识别。
