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[落地生根叶片中黄酮类化合物的分离与鉴定]

[The separation and identification of the flavonoids from the leaves of Bryophyllum pinnatum].

作者信息

Cao Hong, Xia Jie, Xu Diansheng, Lu Bing, Chen Guohao

机构信息

State Key Laboratory of Bioreactor Engineering ECUST, Shanghai 200237.

出版信息

Zhong Yao Cai. 2005 Nov;28(11):988-90.

Abstract

This study investigated the separation of the effective compositions containing quercetin derivatives from the leaves of Bryophyllum pinnatun by solvent extraction of methanol, polar adsorption by porous polymetric resins, mixed solvent extraction of methanol with chloroform and polyamide chromatography. The results indicated that the yield of product containing 51.23% quercetin in the form of quercetin derivatives was 0.2% (W/W). Through the NMR, Mass spectrum, Infrared spectrum, we try to identify the chemical structure of the flavonoids LEF. As a result we can conclude that the chemical structure of the flavonoids from the leaves of Bryophy Ilum pinnatum is C26H28O15 (quercetin3-alpha-L-rha-beta-D-xyl), molecular weight is 580.

摘要

本研究通过甲醇溶剂萃取、多孔聚合物树脂极性吸附、甲醇与氯仿混合溶剂萃取以及聚酰胺色谱法,对落地生根叶片中含槲皮素衍生物的有效成分进行分离。结果表明,以槲皮素衍生物形式存在的、含51.23%槲皮素的产物得率为0.2%(W/W)。通过核磁共振、质谱、红外光谱,我们试图鉴定黄酮类化合物LEF的化学结构。结果我们可以得出结论,落地生根叶片中黄酮类化合物的化学结构为C26H28O15(槲皮素3-α-L-鼠李糖-β-D-木糖),分子量为580。

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