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鸡疱疹病毒1型(传染性喉气管炎病毒):SA-2株的克隆及物理图谱

Gallid herpesvirus 1 (infectious laryngotracheitis virus): cloning and physical maps of the SA-2 strain.

作者信息

Johnson M A, Prideaux C T, Kongsuwan K, Sheppard M, Fahey K J

机构信息

CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria, Australia.

出版信息

Arch Virol. 1991;119(3-4):181-98. doi: 10.1007/BF01310669.

Abstract

Clones representing 90% of the genome of Gallid herpesvirus 1 (infectious laryngotracheitis virus; ILTV) were obtained and used in hybridization experiments to construct EcoRI, KpnI amd SmaI physical maps. The genome was 155 kilobase pairs (kbp) and comprised of a long unique sequence (120 kbp) and a short unique sequence (17 kbp) bounded by repeat sequences each of 9 kbp. An unrelated second pair of repeat sequences was located at 0.67 and 0.88 map untis. A terminal repeat of the unique long region (UL) was also detected, but no isomerization of UL was detected.

摘要

获得了代表鸡疱疹病毒1型(传染性喉气管炎病毒;ILTV)90%基因组的克隆,并将其用于杂交实验以构建EcoRI、KpnI和SmaI物理图谱。该基因组为155千碱基对(kbp),由一个长的单一序列(120 kbp)和一个短的单一序列(17 kbp)组成,其两侧分别为9 kbp的重复序列。另一对不相关的重复序列位于0.67和0.88图谱单位处。还检测到了独特长区域(UL)的末端重复序列,但未检测到UL的异构化。

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