Ghibelli L, Cerella C, Cordisco S, Clavarino G, Marazzi S, De Nicola M, Nuccitelli S, D'Alessio M, Magrini A, Bergamaschi A, Guerrisi V, Porfiri L M
Dipartimento di Biologia, Universita' di Roma Tor Vergata, Rome, Italy.
Apoptosis. 2006 Mar;11(3):359-65. doi: 10.1007/s10495-006-4001-1.
NMR technology has dramatically contributed to the revolution of image diagnostic. NMR apparatuses use combinations of microwaves over a homogeneous strong (1 Tesla) static magnetic field. We had previously shown that low intensity (0.3-66 mT) static magnetic fields deeply affect apoptosis in a Ca2+ dependent fashion (Fanelli et al., 1999 FASEBJ., 13;95-102). The rationale of the present study is to examine whether exposure to the static magnetic fields of NMR can affect apoptosis induced on reporter tumor cells of haematopoietic origin. The impressive result was the strong increase (1.8-2.5 fold) of damage-induced apoptosis by NMR. This potentiation is due to cytosolic Ca2+ overload consequent to NMR-promoted Ca2+ influx, since it is prevented by intracellular (BAPTA-AM) and extracellular (EGTA) Ca2+ chelation or by inhibition of plasma membrane L-type Ca2+ channels. Three-days follow up of treated cultures shows that NMR decrease long term cell survival, thus increasing the efficiency of cytocidal treatments. Importantly, mononuclear white blood cells are not sensitised to apoptosis by NMR, showing that NMR may increase the differential cytotoxicity of antitumor drugs on tumor vs normal cells. This strong, differential potentiating effect of NMR on tumor cell apoptosis may have important implications, being in fact a possible adjuvant for antitumor therapies.
核磁共振技术极大地推动了影像诊断的革命。核磁共振设备在均匀的强（1特斯拉）静磁场中使用微波组合。我们之前已经表明，低强度（0.3 - 66毫特斯拉）静磁场以钙依赖的方式深刻影响细胞凋亡（法内利等人，1999年，《美国实验生物学会联合会杂志》，13卷；95 - 102页）。本研究的基本原理是检验暴露于核磁共振的静磁场是否会影响造血来源的报告肿瘤细胞诱导的细胞凋亡。令人印象深刻的结果是，核磁共振导致损伤诱导的细胞凋亡显著增加（1.8 - 2.5倍）。这种增强作用是由于核磁共振促进钙离子内流导致胞质钙离子过载，因为细胞内（BAPTA - AM）和细胞外（乙二醇双乙醚二胺四乙酸）钙离子螯合或质膜L型钙离子通道的抑制可阻止这种情况。对处理后的培养物进行三天的随访表明，核磁共振降低了细胞的长期存活率，从而提高了细胞杀伤治疗的效率。重要的是，单核白细胞对核磁共振诱导的细胞凋亡不敏感，这表明核磁共振可能会增加抗肿瘤药物对肿瘤细胞与正常细胞的差异细胞毒性。核磁共振对肿瘤细胞凋亡的这种强大的、差异性增强作用可能具有重要意义，实际上它可能是抗肿瘤治疗的一种潜在辅助手段。
