Han Ting, Li Hui-Liang, Hu Yuan, Zhang Qiao-Yan, Huang Bao-Kang, Zheng Han-Chen, Rahman Khalid, Qin Lu-Ping
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Zhong Xi Yi Jie He Xue Bao. 2006 Mar;4(2):194-8. doi: 10.3736/jcim20060217.
To study the chemical constituents of Fructus Xanthii and to determine the contents of total phenolic acids (TPA) in fruits of Xanthium from different populations for evaluating the quality of them.
Components in Fructus Xanthii were isolated and purified by various column chromatographies and the contents of TPA were determined by ultraviolet spectrophotometry with chlorogenic acid (CHA) as reference substance.
Six caffeoylquinic acids along with caffeic acid and ferulic acid were isolated from Fructus Xanthii. The contents of TPA of the samples collected from 29 populations in China varied from 0.31% to 1.44%. Among the samples originated from two species and 1 variety of Xanthium, the contents of TPA in X. sibiricum var. subinerme samples with an average of 0.36% were relatively lower than those in other 2 species. While the content of TPA in Sample 3 collected from Shanghai was 1.44% and the highest among all the samples, and that in Sample 12 from Xinjian of Jiangxi Province was 0.38% and the lowest among the X. sibiricum samples.
5-O-caffeoylquinic acid, 1,4-di-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid were isolated from Xanthium plant for the first time. The difference of contents of TPA in samples from different species and different populations in China was relatively significant. Fructus Xanthii in Shanghai and Sanming of Fujian Province were considered high quality if contents of TPA were used as reference for quality evaluating.
研究苍耳子的化学成分，测定不同居群苍耳果实中总酚酸（TPA）的含量，以评价其质量。
采用多种柱色谱法对苍耳子中的成分进行分离纯化，以绿原酸（CHA）为对照品，采用紫外分光光度法测定TPA的含量。
从苍耳子中分离得到6种咖啡酰奎宁酸以及咖啡酸和阿魏酸。中国29个居群采集的样品中TPA的含量在0.31%至1.44%之间。在源自苍耳属2个种和1个变种的样品中，华东苍耳变种样品中TPA的含量相对较低，平均为0.36%，低于其他2个种。上海采集的3号样品中TPA的含量为1.44%，是所有样品中最高的；江西新建的12号样品中TPA的含量为0.38%，是华东苍耳样品中最低的。
首次从苍耳属植物中分离得到5-O-咖啡酰奎宁酸、1,4-二-O-咖啡酰奎宁酸和4,5-二-O-咖啡酰奎宁酸。中国不同种和不同居群样品中TPA含量差异较为显著。以TPA含量为质量评价指标时，上海和福建三明的苍耳子质量较高。
