Cruz-Rizzolo Roelf J, Horta-Júnior José de Anchieta C, Bittencourt Jackson C, Ervolino Edilson, de Oliveira José Américo, Casatti Cláudio A
Department of Basic Sciences, Araçatuba Campus, UNESP, São Paulo State University, São Paulo, Brazil.
Brain Res. 2006 Apr 14;1083(1):118-33. doi: 10.1016/j.brainres.2006.01.098. Epub 2006 Mar 13.
We studied the distribution of NADPH-diaphorase (NADPH-d) activity in the prefrontal cortex of normal adult Cebus apella monkeys using NADPH-d histochemical protocols. The following regions were studied: granular areas 46 and 12, dysgranular areas 9 and 13, and agranular areas 32 and Oap. NADPH-d-positive neurons were divided into two distinct types, both non-pyramidal. Type I neurons had a large soma diameter (17.24 +/- 1.73 microm) and were densely stained. More than 90% of these neurons were located in the subcortical white matter and infragranular layers. The remaining type I neurons were distributed in the supragranular layers. Type II neurons had a small, round or oval soma (9.83 +/- 1.03 microm), and their staining pattern varied markedly. Type II neurons were distributed throughout the cortex, with their greatest numerical density being observed in layers II and III. In granular areas, the number of type II neurons was up to 20 times that of type I neurons, but this proportion was smaller in agranular areas. Areal density of type II neurons was maximum in the supragranular layers of granular areas and minimum in agranular areas. Statistical analysis revealed that these areal differences were significant when comparing some specific areas. In conclusion, our results indicate a predominance of NADPH-d-positive cells in supragranular layers of granular areas in the Cebus prefrontal cortex. These findings support previous observations on the role of type II neurons as a new cortical nitric oxide source in supragranular cortical layers in primates, and their potential contribution to cortical neuronal activation in advanced mammals.
我们使用NADPH-d组织化学方法研究了成年正常僧帽猴前额叶皮质中NADPH-黄递酶（NADPH-d）活性的分布。研究了以下区域：颗粒区46和12、亚颗粒区9和13以及无颗粒区32和Oap。NADPH-d阳性神经元分为两种不同类型，均为非锥体神经元。I型神经元胞体直径较大（17.24±1.73微米），染色浓密。这些神经元中超过90%位于皮质下白质和颗粒下层。其余I型神经元分布在颗粒上层。II型神经元胞体小，呈圆形或椭圆形（9.83±1.03微米），其染色模式差异明显。II型神经元分布于整个皮质，在II层和III层数量密度最高。在颗粒区，II型神经元数量是I型神经元的20倍，但在无颗粒区该比例较小。II型神经元的面密度在颗粒区的颗粒上层最大，在无颗粒区最小。统计分析表明，比较某些特定区域时，这些区域差异具有显著性。总之，我们的结果表明，在僧帽猴前额叶皮质颗粒区的颗粒上层中，NADPH-d阳性细胞占优势。这些发现支持了之前关于II型神经元作为灵长类动物颗粒上层新的皮质一氧化氮来源的作用及其对高等哺乳动物皮质神经元激活的潜在贡献的观察结果。
