Katz J B, Ludemann L, Pemberton J, Schmerr M J
Vet Microbiol. 1987 Feb;13(2):153-7. doi: 10.1016/0378-1135(87)90040-x.
An indirect immunoperoxidase staining technique was developed for identifying cell cultures infected with bovine virus diarrhea virus. Infected cell monolayers stained intensely while uninfected monolayers remained colorless. Immunoperoxidase staining was as sensitive as direct immunofluorescence in detecting endpoint dilutions of virus suspensions. Using the immunoperoxidase technique, infected monolayers were detectable by macroscopic, as well as microscopic, observation.
已开发出一种间接免疫过氧化物酶染色技术，用于鉴定感染牛病毒性腹泻病毒的细胞培养物。感染的细胞单层染色强烈，而未感染的单层仍保持无色。在检测病毒悬液的终点稀释度时，免疫过氧化物酶染色与直接免疫荧光一样灵敏。使用免疫过氧化物酶技术，通过宏观以及微观观察均可检测到感染的单层。
