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瘟病毒是梅迪-维斯纳病病毒和山羊关节炎-脑炎病毒毒株中的常见污染物。

Pestivirus is a common contaminant in maedi-visna and caprine arthritis-encephalitis virus stocks.

作者信息

Heckert R A, Power C A, Briscoe M R

机构信息

Agriculture Canada, Animal Disease Research Institute, Nepean, Ontario.

出版信息

Can J Vet Res. 1992 Oct;56(4):370-2.

Abstract

Eight different laboratory stocks of maedi-visna or caprine arthritis-encephalitis virus were examined for the presence of pestiviruses by a fixed-cell immunoperoxidase assay with polyclonal and monoclonal antibodies. All of the viral stocks examined were found to contain noncytopathic pestivirus contaminants. The panel of monoclonal antibodies could not type the isolates as being more related to bovine virus diarrhea virus or border disease virus. However, the results did indicate that all isolates were not the same, except for two from the same laboratory where the source of pestivirus contamination may have been common.

摘要

通过使用多克隆抗体和单克隆抗体的固定细胞免疫过氧化物酶测定法,对八种不同的梅迪-维斯纳病或山羊关节炎-脑炎病毒实验室毒株进行了瘟病毒检测。所有检测的病毒毒株均被发现含有非细胞病变性瘟病毒污染物。单克隆抗体组无法将这些分离株鉴定为与牛病毒性腹泻病毒或边界病病毒关系更密切。然而,结果确实表明,除了来自同一实验室的两个毒株(其瘟病毒污染来源可能相同)外,所有分离株都不相同。

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Peroxidase-labeled primary antibody method for detection of pestivirus contamination in cell cultures.
J Virol Methods. 1989 Oct;26(1):119-24. doi: 10.1016/0166-0934(89)90080-3.

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