Raschke W C, Ralph P, Watson J, Sklar M, Coon H
J Natl Cancer Inst. 1975 May;54(5):1249-53. doi: 10.1093/jnci/54.5.1249.
Spleen cell cultures stimulated to DNA synthesis by antigen or mitogen were infected with Abelson virus, a C-type RNA virus inducing nonthymic lymphomas in mice. After 3 days the cells were transferred to mice and caused 100 percent incidence of lymphomas in as few as 29 days. That a number of the tumors were of donor origin, as shown by female karyotypes in recipient male mice, indicated that cells infected by virus in vitro were transformed. The process depended upon both virus and stimulation of lymphocytes in culture. Lymphoid tumors did not develop in mice receiving cells from virus-infected cultures not exposed to antigen or mitogen.
用抗原或丝裂原刺激进行DNA合成的脾细胞培养物，被Abelson病毒感染，Abelson病毒是一种能在小鼠中诱发非胸腺淋巴瘤的C型RNA病毒。3天后，将这些细胞移植到小鼠体内，在短短29天内就导致了100%的淋巴瘤发病率。如受体雄性小鼠中的雌性核型所示，许多肿瘤来自供体，这表明体外被病毒感染的细胞发生了转化。这个过程既依赖于病毒，也依赖于培养中淋巴细胞的刺激。接受未接触抗原或丝裂原的病毒感染培养物中的细胞的小鼠不会发生淋巴瘤。
