通过羟基磷灰石色谱法将人血清脂蛋白分离为三大类。

Separation of human serum lipoproteins into three major classes by hydroxyapatite chromatography.

作者信息

Matsumoto U, Nakayama H, Shibusawa Y, Niimura T

机构信息

Division of Analytical Chemistry, Tokyo College of Pharmacy, Japan.

出版信息

J Chromatogr. 1991 May 3;566(1):67-76. doi: 10.1016/0378-4347(91)80111-o.

DOI:10.1016/0378-4347(91)80111-o

PMID:1653259

Abstract

The separation of normolipidemic male serum lipoprotein fraction, prepared by ultracentrifugal flotation, was studied on hydroxyapatite columns. Potassium phosphate buffers in the pH range 5.6-7.4 were evaluated as eluents. The three main classes of the lipoproteins (high density, low density and very low density) can be separated on the Tiselius-type hydroxyapatite (Bio-Gel HTP DNA grade) column by elution with 75, 250 and 300 mM potassium phosphate buffer (pH 7.4), respectively.

摘要

对通过超速离心浮选法制备的正常血脂男性血清脂蛋白组分在羟基磷灰石柱上的分离情况进行了研究。评估了pH值范围为5.6 - 7.4的磷酸钾缓冲液作为洗脱剂的效果。三种主要类型的脂蛋白（高密度、低密度和极低密度）可分别通过用75、250和300 mM磷酸钾缓冲液（pH 7.4）洗脱，在蒂塞利乌斯型羟基磷灰石（Bio-Gel HTP DNA级）柱上实现分离。