Okazaki M, Ohno Y, Hara I
J Chromatogr. 1980 Dec 12;221(2):257-64. doi: 10.1016/s0378-4347(00)84310-8.
A new application of high-performance aqueous gel permeation chromatography was developed for the analysis of human serum lipoproteins. A good combination of columns (TSK GEL, type PW and type SW) was found for the separation of serum lipoproteins: very low-density lipoprotein, low-density lipoprotein and high-density lipoproteins. Analyses of serum lipoproteins from individual normal subjects and pathological subjects were performed by this combination of columns. The effects of pH and salt concentration fo the eluent on the separation of lipoproteins were also investigated.
开发了一种用于分析人血清脂蛋白的高性能水性凝胶渗透色谱新应用。发现了用于分离血清脂蛋白（极低密度脂蛋白、低密度脂蛋白和高密度脂蛋白）的柱（TSK GEL，PW型和SW型）的良好组合。通过这种柱组合对个体正常受试者和病理受试者的血清脂蛋白进行了分析。还研究了洗脱液的pH值和盐浓度对脂蛋白分离的影响。
