Ullah Ihsan, Subhan Fazal, Ayaz Muhammad, Shah Rehmat, Ali Gowhar, Haq Ikram Ul, Ullah Sami
Department of Pharmacy, University of Swabi, Swabi, Pakistan.
Department of Pharmacy, University of Peshawar, Peshawar, Pakistan.
BMC Complement Altern Med. 2015 Feb 26;15:34. doi: 10.1186/s12906-015-0556-0.
Zingiber officinale (ZO, family Zingiberaceae) has been reported for its antiemetic activity against cancer chemotherapy induced emesis in animal models and in clinics. Current study was designed to investigate ZO for potential usefulness against cisplatin induced vomiting in pigeon and its effects on central and peripheral neurotransmitters involved in the act of vomiting.
Zingiber officinale acetone fraction (ZO-ActFr) was investigated for attenuation of emesis induced by cisplatin in healthy pigeons. Neurotransmitters DA, 5HT and their metabolites DOPAC, HVA and 5HIAA were analyzed using High Performance Liquid Chromatography system coupled with electrochemical detector in area postrema, brain stem and intestine. Antiemetic effect of ZO-ActFr was correlated with central and intestinal neurotransmitters levels in pigeon.
Cisplatin (7 mg/kg i.v.) induced emesis without lethality upto the observation period. ZO-ActFr (25, 50 & 100 mg/kg) attenuated cisplatin induced emesis ~ 44.18%, 58.13% (P < 0.05) and 27.9%, respectively; the reference drug, metoclopramide (MCP; 30 mg/kg), produced ~ 48.83% reduction (P < 0.05). ZO-ActFr reduced (P < 0.05 - 0.001) 5-hydroxytryptamine (5HT) concentration in the area postrema, brain stem and intestine at 3(rd) hour of cisplatin administration, while at the 18(th) hour ZO treatments attenuated the dopamine upsurge (P < 0.001) caused by cisplatin in the area postrema and 5HT concentration (P < 0.01 - 0.001) in the brain stem and intestine. ZO treatments alone did not altered the basal neurotransmitters and their metabolites in the brain areas and intestine.
The behavioral study verify the antiemetic profile of ZO against cisplatin induced emesis in the pigeon, where central and peripheral neural evidences advocate the involvement of serotonergic mechanism at initial time point (3(rd) hr), while the later time point (18(th) hr) is associated with serotonergic and dopaminergic component in the mediation of its antiemetic effect.
姜科植物姜（Zingiber officinale，ZO）在动物模型和临床研究中已被报道具有对抗癌症化疗引起呕吐的止吐活性。本研究旨在探究姜对顺铂诱导家鸽呕吐的潜在作用及其对呕吐相关中枢和外周神经递质的影响。
研究姜丙酮提取物（ZO-ActFr）对健康家鸽顺铂诱导呕吐的缓解作用。在最后区、脑干和肠道中，使用高效液相色谱系统结合电化学检测器分析神经递质多巴胺（DA）、5-羟色胺（5HT）及其代谢产物3,4-二羟基苯乙酸（DOPAC）、高香草酸（HVA）和5-羟吲哚乙酸（5HIAA）。将ZO-ActFr的止吐作用与家鸽中枢和肠道神经递质水平进行关联分析。
在观察期内，静脉注射7mg/kg顺铂可诱导家鸽呕吐但无致死性。ZO-ActFr（25、50和100mg/kg）分别使顺铂诱导的呕吐减少约44.18%、58.13%（P<0.05）和27.9%；阳性对照药胃复安（MCP，30mg/kg）使呕吐减少约48.83%（P<0.05）。在顺铂给药后第3小时，ZO-ActFr降低了（P<0.05 - 0.001）最后区、脑干和肠道中5-羟色胺（5HT）的浓度；而在第18小时，ZO处理减弱了顺铂引起的最后区多巴胺激增（P<0.001）以及脑干和肠道中5HT浓度的升高（P<0.01 - 0.001）。单独使用ZO处理未改变脑区和肠道中基础神经递质及其代谢产物的水平。
行为学研究证实了姜对顺铂诱导家鸽呕吐具有止吐作用，其中枢和外周神经证据表明在初始时间点（第3小时）5-羟色胺能机制参与其中，而在后期时间点（第18小时）其止吐作用的介导涉及5-羟色胺能和多巴胺能成分。
Zotero 插件