利用荧光素酶基因作为生物传感器来研究消化道中的细菌生理学。
Use of luciferase genes as biosensors to study bacterial physiology in the digestive tract.
作者信息
Corthier G, Delorme C, Ehrlich S D, Renault P
机构信息
Unite d'Ecologie et de Physiologie Digestive-FBI, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
出版信息
Appl Environ Microbiol. 1998 Jul;64(7):2721-2. doi: 10.1128/AEM.64.7.2721-2722.1998.
Abstract
A method based on the use of the bacterial luciferase genes was developed in order to investigate Lactococcus lactis gene expression in the mouse digestive tract. Germfree mice were monoassociated with different strains containing transcriptional fusions of promoters with the luciferase genes. Our results demonstrate that this method is readily applicable to the study of promoter strength and physiology of bacteria in the digestive tract.
摘要
为了研究乳酸乳球菌在小鼠消化道中的基因表达，开发了一种基于使用细菌荧光素酶基因的方法。无菌小鼠与含有启动子与荧光素酶基因转录融合体的不同菌株进行单关联。我们的结果表明，该方法易于应用于研究消化道中细菌的启动子强度和生理学。
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