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利用荧光素酶基因作为生物传感器来研究消化道中的细菌生理学。

Use of luciferase genes as biosensors to study bacterial physiology in the digestive tract.

作者信息

Corthier G, Delorme C, Ehrlich S D, Renault P

机构信息

Unite d'Ecologie et de Physiologie Digestive-FBI, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.

出版信息

Appl Environ Microbiol. 1998 Jul;64(7):2721-2. doi: 10.1128/AEM.64.7.2721-2722.1998.

DOI:10.1128/AEM.64.7.2721-2722.1998
PMID:9647856
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC106452/
Abstract

A method based on the use of the bacterial luciferase genes was developed in order to investigate Lactococcus lactis gene expression in the mouse digestive tract. Germfree mice were monoassociated with different strains containing transcriptional fusions of promoters with the luciferase genes. Our results demonstrate that this method is readily applicable to the study of promoter strength and physiology of bacteria in the digestive tract.

摘要

为了研究乳酸乳球菌在小鼠消化道中的基因表达，开发了一种基于使用细菌荧光素酶基因的方法。无菌小鼠与含有启动子与荧光素酶基因转录融合体的不同菌株进行单关联。我们的结果表明，该方法易于应用于研究消化道中细菌的启动子强度和生理学。

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本文引用的文献

1
REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.枯草芽孢杆菌转化的要求。
J Bacteriol. 1961 May;81(5):741-6. doi: 10.1128/jb.81.5.741-746.1961.
2
Use of controlled luciferase expression to monitor chemicals affecting protein synthesis.利用受控的荧光素酶表达来监测影响蛋白质合成的化学物质。
Appl Environ Microbiol. 1995 Aug;61(8):2981-9. doi: 10.1128/aem.61.8.2981-2989.1995.
3
Improved medium for lactic streptococci and their bacteriophages.用于乳酸链球菌及其噬菌体的改良培养基。
Appl Microbiol. 1975 Jun;29(6):807-13. doi: 10.1128/am.29.6.807-813.1975.
4
Plasmid vectors for gram-positive bacteria switching from high to low copy number.用于革兰氏阳性菌的从高拷贝数转换为低拷贝数的质粒载体。
Gene. 1996 Dec 12;183(1-2):175-82. doi: 10.1016/s0378-1119(96)00554-9.
5
Lactic acid bacteria as vaccine delivery vehicles.作为疫苗递送载体的乳酸菌。
Antonie Van Leeuwenhoek. 1996 Oct;70(2-4):317-30. doi: 10.1007/BF00395939.
6
Lactococcus lactis and stress.乳酸乳球菌与应激
Antonie Van Leeuwenhoek. 1996 Oct;70(2-4):243-51. doi: 10.1007/BF00395935.
7
Biotherapeutic agents. A neglected modality for the treatment and prevention of selected intestinal and vaginal infections.
JAMA. 1996 Mar 20;275(11):870-6. doi: 10.1001/jama.275.11.870.
8
Potential of using lactic acid bacteria for therapy and immunomodulation in man.乳酸菌在人类治疗和免疫调节中的应用潜力。
FEMS Microbiol Rev. 1993 Sep;12(1-3):207-20. doi: 10.1111/j.1574-6976.1993.tb00019.x.
9
The use of bacterial luciferase genes as reporter genes in Lactococcus: regulation of the Lactococcus lactis subsp. lactis lactose genes.细菌荧光素酶基因在乳酸乳球菌中作为报告基因的应用：乳酸乳球菌乳亚种乳糖基因的调控
J Gen Microbiol. 1993 Jul;139(7):1495-501. doi: 10.1099/00221287-139-7-1495.
10
The Lactococcus lactis sex-factor aggregation gene cluA.乳酸乳球菌性别因子聚集基因cluA。
Mol Microbiol. 1994 May;12(4):655-63. doi: 10.1111/j.1365-2958.1994.tb01053.x.