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1
A Method for Purification of Clostridium perfringens Phospholipase C from Recombinant Bacillus subtilis Cells.从重组枯草芽孢杆菌细胞中纯化产气荚膜梭菌磷脂酶 C 的方法。
Appl Environ Microbiol. 1995 Nov;61(11):4114-5. doi: 10.1128/aem.61.11.4114-4115.1995.
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6
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BMC Microbiol. 2017 Jul 4;17(1):150. doi: 10.1186/s12866-017-1051-1.
7
Expression from the Clostridium perfringens cpe promoter in C. perfringens and Bacillus subtilis.产气荚膜梭菌cpe启动子在产气荚膜梭菌和枯草芽孢杆菌中的表达。
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8
Antibacterial activity of Bacillus species-derived surfactin on Brachyspira hyodysenteriae and Clostridium perfringens.芽孢杆菌属来源的表面活性素对猪痢疾短螺旋体和产气荚膜梭菌的抗菌活性。
AMB Express. 2019 Nov 21;9(1):188. doi: 10.1186/s13568-019-0914-2.
9
Small cytoplasmic RNA (scRNA) gene from Clostridium perfringens can replace the gene for the Bacillus subtilis scRNA in both growth and sporulation.产气荚膜梭菌的小细胞质RNA（scRNA）基因在生长和孢子形成过程中均可替代枯草芽孢杆菌scRNA的基因。
Microbiology (Reading). 1995 Nov;141 ( Pt 11):2965-75. doi: 10.1099/13500872-141-11-2965.
10
Role of alpha-toxin in Clostridium perfringens infection determined by using recombinants of C. perfringens and Bacillus subtilis.通过使用产气荚膜梭菌和枯草芽孢杆菌的重组体确定α毒素在产气荚膜梭菌感染中的作用。
Infect Immun. 1994 Nov;62(11):5032-9. doi: 10.1128/iai.62.11.5032-5039.1994.

本文引用的文献

1
Protein secretion in Bacillus species.芽孢杆菌属中的蛋白质分泌
Microbiol Rev. 1993 Mar;57(1):109-37. doi: 10.1128/mr.57.1.109-137.1993.
2
Bacterial phospholipases C.细菌磷脂酶C
Microbiol Rev. 1993 Jun;57(2):347-66. doi: 10.1128/mr.57.2.347-366.1993.
3
Regulation of Clostridium perfringens alpha-toxin-activated phospholipase C in rabbit erythrocyte membranes.兔红细胞膜中产气荚膜梭菌α毒素激活的磷脂酶C的调节
Infect Immun. 1994 Feb;62(2):717-21. doi: 10.1128/iai.62.2.717-721.1994.
4
Role of alpha-toxin in Clostridium perfringens infection determined by using recombinants of C. perfringens and Bacillus subtilis.通过使用产气荚膜梭菌和枯草芽孢杆菌的重组体确定α毒素在产气荚膜梭菌感染中的作用。
Infect Immun. 1994 Nov;62(11):5032-9. doi: 10.1128/iai.62.11.5032-5039.1994.
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Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases.枯草芽孢杆菌细胞外碱性和中性蛋白酶缺陷型双突变体的构建。
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Purification of Clostridium perfringens phospholipase C (alpha-toxin) by affinity chromatography on agarose-linked egg-yolk lipoprotein.通过琼脂糖连接的蛋黄脂蛋白亲和层析法纯化产气荚膜梭菌磷脂酶C（α毒素）
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Stable hyper-production of Escherichia coli beta-lactamase by Bacillus subtilis grown on a 0.5 M succinate-medium using a B. subtilis alpha-amylase secretion vector.
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Assay methods for alpha toxin from Clostridium perfringens: phospholipase C.
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Lethal effects and cardiovascular effects of purified alpha- and theta-toxins from Clostridium perfringens.产气荚膜梭菌纯化α毒素和θ毒素的致死效应及心血管效应
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Cloning and sequencing of a phospholipase C gene of Clostridium perfringens.产气荚膜梭菌磷脂酶C基因的克隆与测序
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从重组枯草芽孢杆菌细胞中纯化产气荚膜梭菌磷脂酶 C 的方法。

A Method for Purification of Clostridium perfringens Phospholipase C from Recombinant Bacillus subtilis Cells.

出版信息

Appl Environ Microbiol. 1995 Nov;61(11):4114-5. doi: 10.1128/aem.61.11.4114-4115.1995.

DOI:10.1128/aem.61.11.4114-4115.1995
PMID:16535170
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1388606/
Abstract

We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture.

摘要

我们开发了一种从重组枯草芽孢杆菌细胞培养物中纯化产气荚膜梭菌磷脂酶 C 的方法。该方法包括三个纯化步骤，我们能够从 800 毫升培养物中获得 6.2 毫克纯的磷脂酶 C。