Appl Environ Microbiol. 1996 May;62(5):1630-5. doi: 10.1128/aem.62.5.1630-1635.1996.
A collection of about 200 actinomycete strains was screened for the ability to grow on fragmented Phytophthora mycelium and to produce metabolites that inhibit Phytophthora growth. Thirteen strains were selected, and all produced (beta)-1,3-, (beta)-1,4-, and (beta)-1,6-glucanases. These enzymes could hydrolyze glucans from Phytophthora cell walls and cause lysis of Phytophthora cells. These enzymes also degraded other glucan substrates, such as cellulose, laminarin, pustulan, and yeast cell walls. Eleven strains significantly reduced the root rot index when inoculated on raspberry plantlets.
从约 200 株放线菌菌株中筛选出能够利用碎化的疫霉菌丝生长并产生抑制疫霉生长的代谢产物的菌株。选择了 13 株菌株，所有菌株都产生了 (β)-1,3-、(β)-1,4- 和 (β)-1,6-葡聚糖酶。这些酶能够水解疫霉菌壁中的葡聚糖并导致疫霉菌细胞裂解。这些酶还降解其他的葡聚糖底物，如纤维素、昆布多糖、普鲁兰和酵母细胞壁。当接种在覆盆子幼苗上时，有 11 株菌株显著降低了根腐病指数。
