甲苯 2-单加氧酶依赖的伯克霍尔德氏菌 G4/PR1 在乙醚上的生长。
Toluene 2-Monooxygenase-Dependent Growth of Burkholderia cepacia G4/PR1 on Diethyl Ether.
出版信息
Appl Environ Microbiol. 1997 Apr;63(4):1606-9. doi: 10.1128/aem.63.4.1606-1609.1997.
Abstract
Aerobic bacterial growth on aromatic hydrocarbons typically requires oxygenase enzymes, which are known to fortuitously oxidize nongrowth substrates. In this study, we found that oxidation of diethyl ether by toluene 2-monooxygenase supported more rapid growth of Burkholderia cepacia G4/PR1 than did the aromatic substrates n-propylbenzene and o-xylene. The wild-type Burkholderia cepacia G4 failed to grow on diethyl ether. Purified toluene 2-monooxygenase protein components oxidized diethyl ether stoichiometrically to ethanol and acetaldehyde. Butyl methyl ether, diethyl sulfide, and 2-chloroethyl ethyl ether were oxidized by B. cepacia G4/PR1.
摘要
芳香烃上的需氧细菌生长通常需要加氧酶，已知这些酶会偶然氧化非生长基质。在这项研究中，我们发现甲苯 2-单加氧酶氧化乙醚支持比芳香族基质正丙苯和邻二甲苯更快速的伯克霍尔德氏菌生长。野生型伯克霍尔德氏菌 G4 不能在乙醚上生长。纯化的甲苯 2-单加氧酶蛋白成分将乙醚化学计量地氧化为乙醇和乙醛。丁基甲基醚、二乙基硫醚和 2-氯乙基乙基醚被 G4/PR1 氧化。
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