Wang Hsing-Won, Kao Chuan-Hsiang, Chu Yueng-Hsiang
Department of Otolaryngology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Am J Rhinol. 2006 Jan-Feb;20(1):43-7.
The calculation of cell planar surface area, successfully used for glomerular mesangial cell-altered contractility, also is a reasonable methodology to assess nasal mucosal contractility. A simple technique that works particularly well with vasoconstriction-inducing agents has been introduced to test the effects of several drugs on patient turbinate nasal mucosa in vitro. This report will introduce this technique and present some of the initial findings.
We tested epinephrine, norepinephrine, pseudoephedrine HCl, and methoxamine, respectively, for effectiveness as a vasoconstricting drug. We also tested the effectiveness of verapamil as a vasodilating drug. A piece of nasal mucosa approximately 3 x 4 mm in size was mounted with a 27-gauge needle and submersed in a 4-mL muscle bath in a Petri dish at room temperature. The bath consisted of 4 mL of Kreb's solution. We recorded the decrease of nasal mucosa planar surface area in response to the application of vasoconstricting agents, as measured using a digital microscope connected to a Pentium III with morphometric software, as the estimated degree of contraction. A photograph of the same mucosa was taken every 3 minutes for a total period of 18 minutes.
Nasal mucosa that was mounted in a muscle bath in a Petri dish and treated with a small dose of a nasal decongestant was l observed to contract. Drug dosage determined the degree of nasal mucosa contraction. Nasal mucosa similarly prepared but treated with a vasodilating agent, such as verapamil, exhibited a relaxation response only when pretreated with a vasoconstricting agent, such as methoxamine. Both vasoconstricting and vasodilating drugs induce dose-related responses in planar surface calculation.
In this study, drug dosage determined the degree of nasal mucosa contraction. We also found that the rate of contraction and percent of contraction were dose dependent. We believe this new method will prove useful in studying the effects of drugs on nasal blood vessels.
细胞平面表面积的计算已成功用于评估肾小球系膜细胞改变的收缩性，也是评估鼻黏膜收缩性的合理方法。已引入一种特别适用于血管收缩诱导剂的简单技术，以在体外测试几种药物对患者鼻甲鼻黏膜的作用。本报告将介绍该技术并展示一些初步研究结果。
我们分别测试了肾上腺素、去甲肾上腺素、盐酸伪麻黄碱和甲氧明作为血管收缩药物的有效性。我们还测试了维拉帕米作为血管舒张药物的有效性。将一块大小约为3×4毫米的鼻黏膜用27号针头固定，并在室温下浸入培养皿中的4毫升肌肉浴中。浴液由4毫升克雷布斯溶液组成。我们记录了使用连接到奔腾III并带有形态测量软件的数字显微镜测量的，鼻黏膜平面表面积因应用血管收缩剂而减少的情况，并将其作为估计的收缩程度。每隔3分钟拍摄同一块黏膜的照片，共拍摄18分钟。
观察到固定在培养皿中的肌肉浴中并用小剂量鼻减充血剂处理的鼻黏膜会收缩。药物剂量决定了鼻黏膜收缩的程度。同样制备但用血管舒张剂（如维拉帕米）处理的鼻黏膜，仅在先用血管收缩剂（如甲氧明）预处理时才表现出舒张反应。血管收缩药和血管舒张药在平面表面积计算中均诱导剂量相关反应。
在本研究中，药物剂量决定了鼻黏膜收缩的程度。我们还发现收缩速率和收缩百分比与剂量有关。我们相信这种新方法将被证明对研究药物对鼻血管的作用有用。
