Castoldi Mirco, Schmidt Sabine, Benes Vladimir, Noerholm Mikkel, Kulozik Andreas E, Hentze Matthias W, Muckenthaler Martina U
Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Germany.
RNA. 2006 May;12(5):913-20. doi: 10.1261/rna.2332406. Epub 2006 Mar 15.
MicroRNAs represent a class of short (approximately 22 nt), noncoding regulatory RNAs involved in development, differentiation, and metabolism. We describe a novel microarray platform for genome-wide profiling of mature miRNAs (miChip) using locked nucleic acid (LNA)-modified capture probes. The biophysical properties of LNA were exploited to design probe sets for uniform, high-affinity hybridizations yielding highly accurate signals able to discriminate between single nucleotide differences and, hence, between closely related miRNA family members. The superior detection sensitivity eliminates the need for RNA size selection and/or amplification. MiChip will greatly simplify miRNA expression profiling of biological and clinical samples.
微小RNA代表一类短的（约22个核苷酸）非编码调节RNA，参与发育、分化和代谢过程。我们描述了一种新型微阵列平台（miChip），用于使用锁核酸（LNA）修饰的捕获探针进行全基因组成熟微小RNA（miRNA）分析。利用LNA的生物物理特性设计探针组，以实现均匀、高亲和力的杂交，产生能够区分单核苷酸差异的高度准确信号，从而区分密切相关的miRNA家族成员。卓越的检测灵敏度无需进行RNA大小选择和/或扩增。MiChip将极大地简化生物和临床样品的miRNA表达分析。
