Lemon S M, Amphlett E, Sangar D
Department of Medicine, University of North Carolina, Chapel Hill 27599-7030.
J Virol. 1991 Oct;65(10):5636-40. doi: 10.1128/JVI.65.10.5636-5640.1991.
High concentrations of either trypsin or chymotrypsin caused nearly complete cleavage of capsid protein VP2 of hepatitis A virus but did not significantly reduce the infectivity, thermostability, or antigenicity of the virus. Chymotrypsin also had a lesser effect on VP1. These findings indicate the presence of a protease-accessible VP2 surface site which neither contributes significantly to the dominant antigenic site nor plays a role in the attachment of the virus to putative cell receptors.
高浓度的胰蛋白酶或胰凝乳蛋白酶几乎可导致甲型肝炎病毒衣壳蛋白VP2完全裂解，但不会显著降低病毒的感染性、热稳定性或抗原性。胰凝乳蛋白酶对VP1的作用也较小。这些发现表明存在一个蛋白酶可及的VP2表面位点，该位点对主要抗原位点没有显著贡献，在病毒与假定细胞受体的附着过程中也不起作用。
