Alves V A F, Castelo A, Filho A L, Vianna M R, Taromaru E, Namiyama G, Lorincz A, Dores G B D
School of Medicine, São Paulo University, Brazil.
Cytopathology. 2006 Apr;17(2):86-93. doi: 10.1111/j.1365-2303.2006.00279.x.
To evaluate the performance of a new, manual, simplified liquid-based system, DNA-Citoliq (Digene Brasil), employed under routine conditions as compared to conventional smears collected from six collaborating private laboratories.
A panel of cytopathologists, who served as the gold standard diagnosis, adjudicated discordant opinions.
Of 3206 pairs of slides considered valid for comparison, there were 3008 in full agreement (93.8%), 112 (3.5%) with one diagnostic category discrepancies, and 86 (2.7%) discordant cases. Among the 288 borderline+ by either method, DNA-Citoliq detected abnormalities in 243 (84.4%), and conventional smears (CS) detected abnormalities in 178 (61.8%) (McNemar test, P < 0.000), a 36.5% increased detection of borderline+ cases.
For mild dyskaryosis, DNA-Citoliq detected 176 cases and CS 125 cases (McNemar test, P < 0.000); and for moderate+severe dyskaryosis 66 versus 32 cases respectively (McNemar test, P < 0.000).
评估一种新型手动简化液基系统DNA-Citoliq（迪基因巴西公司）在常规条件下的性能，并与从六个合作私立实验室收集的传统涂片进行比较。
一组细胞病理学家作为金标准诊断，对不一致的意见进行裁决。
在3206对被认为可用于比较的玻片标本中，完全一致的有3008对（93.8%），有一个诊断类别差异的有112对（3.5%），不一致的病例有86对（2.7%）。在两种方法检测出的288例临界+病例中，DNA-Citoliq检测到异常的有243例（84.4%），传统涂片（CS）检测到异常的有178例（61.8%）（McNemar检验，P<0.000），临界+病例的检测率提高了36.5%。
对于轻度核异质，DNA-Citoliq检测到176例，CS检测到125例（McNemar检验，P<0.000）；对于中度+重度核异质，分别为66例和32例（McNemar检验，P<0.000）。
