McCann J, Spingarn N E, Kobori J, Ames B N
Proc Natl Acad Sci U S A. 1975 Mar;72(3):979-83. doi: 10.1073/pnas.72.3.979.
We described previously a simple test on petri plates for detecting chemical carcinogens as mutagens, using an especially sensitive set of bacterial strains to detect mutagenic acitivty and a mammalian liver extract for carcinogen activity. We now extend the utility of the method by introducing two new bacterial strains which can detect with great sensitivity many carcinogens which we did not detect before or detected with less sensitivity. Among these carcinogens are aflatoxin B-1, sterigmatocystin, benzyl chloride, benzo[a]-pyrene, 7,12-dimethylbenzanthracene, 1'-acetoxysafrole, and the nitrofuran food additive furylfuramide (AF-2). The new strains TA100 and TA98 contain an R factor plasmid, pKM101, in our standard tester strains TA1535 and TA1538. The R factor increases mutagenesis with certain mutagens, but not others. We present evidence that the mutagens that become more effective work through an error-prone recombinational repair.
我们之前描述过一种在培养皿上进行的简单测试，用于检测作为诱变剂的化学致癌物，该测试使用一组特别敏感的细菌菌株来检测诱变活性，并使用哺乳动物肝脏提取物来检测致癌活性。现在，我们通过引入两种新的细菌菌株来扩展该方法的实用性，这两种新菌株能够极其灵敏地检测出许多我们之前未检测到或检测灵敏度较低的致癌物。这些致癌物包括黄曲霉毒素B-1、杂色曲霉素、苄基氯、苯并[a]芘、7,12-二甲基苯并蒽、1'-乙酰氧基黄樟素以及硝基呋喃类食品添加剂呋啶酰胺（AF-2）。新菌株TA100和TA98在我们的标准测试菌株TA1535和TA1538中含有一种R因子质粒pKM101。R因子会增加某些诱变剂的诱变作用，但对其他诱变剂则不然。我们提供的证据表明，那些变得更有效的诱变剂是通过易错重组修复起作用的。
