病毒诱导的迁移抑制因子与传统的淋巴细胞源性迁移抑制因子的生物学及抗原相似性。
Biologic and antigenic similarity of virus-induced migration inhibition factor to conventional, lymphocyte-derived migration inhibition factor.
作者信息
Yoshida T, Bigazzi P, Cohen S
出版信息
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1641-4. doi: 10.1073/pnas.72.4.1641.
Macrophage migration inhibition factor (MIF) is one of a class of lymphocyte-derived mediator substances (lymphokines) which plays a role in the mechanism of cellular immunity. A variety of other soluble factors produced by non-lymphoid cells have been shown to have effects on macrophage mobility similar to that of MIF. In the present study we demonstrate that one such factor, (MIFV) derived from simian virus 40-infected kidney cells in culture, has several other properties in common with lymphocyte-derived MIF (MIFL), MIFV can be adsorbed on Sepharose bead columns conjugated with an antiserum prepared against MIFL, demonstrating at least some antigenic similarity. Moreover, MIFV can substitute for MIFL in an in vivo system involving the suppression of cutaneous manifestations of cellular immunity by intravenous injection of the lymphokine. These observations, taken in conjunction with the similarity of the in vitro effect of MIFV and MIFL, and their similar chromatographic behavior, suggest that MIFV and MIFL may be identical molecular species.
巨噬细胞移动抑制因子（MIF）是一类淋巴细胞衍生的介质物质（淋巴因子）之一，在细胞免疫机制中发挥作用。已证明非淋巴细胞产生的多种其他可溶性因子对巨噬细胞迁移具有与MIF类似的作用。在本研究中，我们证明了一种这样的因子，即从培养的感染猿猴病毒40的肾细胞中获得的（MIFV），与淋巴细胞衍生的MIF（MIFL）具有其他几个共同特性。MIFV可以吸附在与针对MIFL制备的抗血清偶联的琼脂糖珠柱上，这表明至少存在一些抗原相似性。此外，在涉及通过静脉注射淋巴因子抑制细胞免疫的皮肤表现的体内系统中，MIFV可以替代MIFL。这些观察结果，结合MIFV和MIFL体外作用的相似性及其相似的色谱行为，表明MIFV和MIFL可能是相同的分子种类。
Zotero 插件