一种用于免疫荧光检测感染人成纤维细胞中SV40 T抗原的改良固定方法。
An improved method of fixation for immunofluorescent detection of SV40 T-antigen in infected human fibroblasts.
作者信息
Kaplan M M, Giard D J, Blattner W A, Lubiniecki A S, Fraumeni J F
出版信息
Proc Soc Exp Biol Med. 1975 Mar;148(3):660-4. doi: 10.3181/00379727-148-38605.
PMID:165531
Abstract
Improved fixation for immunofluorescence staining in the SV40 T-antigen test has been developed utilizing ethanol in place of acetone. The modified method improves resolution, results in smaller variability of cell numbers, and reduces the time of assay, while maintaining its sensitivity in discriminating between normal and cancer prone groups.
摘要
在SV40 T抗原检测中，已开发出一种改进的免疫荧光染色固定方法，该方法使用乙醇替代丙酮。改进后的方法提高了分辨率，使细胞数量的变异性更小，并缩短了检测时间，同时保持了其在区分正常组和癌症易感组方面的敏感性。
Zotero 插件